ArticleBMC genomics2016
Genome analysis and avirulence gene cloning using a high-density RADseq linkage map of the flax rust fungus, Melampsora lini.
Article in BMC genomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 80 citations in OpenAlex.
- Avirulence genes identified through linkage mapping and region-specific association studies in the wheat leaf rust pathogen Puccinia triticina.BMC genomics · 2026Article
- EffectorFrontiers in microbiology · 2025Article
- A high-resolution haplotype collection uncovers somatic hybridization, recombination and intercontinental movement in oat crown rust.PLoS genetics · 2024Article
- Use of the Puccinia sorghi haustorial transcriptome to identify and characterize AvrRp1-D recognized by the maize Rp1-D resistance protein.PLoS pathogens · 2024Article
- Variability and functional characterization of the Phakopsora pachyrhizi Egh16-like effectors.Genetics and molecular biology · 2024Article
- Overview and Management of the Most Common Eukaryotic Diseases of Flax (Plants (Basel, Switzerland) · 2023Review
- Transcriptome analysis ofFrontiers in microbiology · 2023Article
- Plant and pathogen genomics: essential approaches for stem rust resistance gene stacks in wheat.Frontiers in plant science · 2023Review
- Advances in Fungal Elicitor-Triggered Plant Immunity.International journal of molecular sciences · 2022Review
- Current Status and Future Perspectives of Genomics Research in the Rust Fungi.International journal of molecular sciences · 2022Review
- Thirty years of resistance: Zig-zag through the plant immune system.The Plant cell · 2022Review
- Characterization of two conserved cell death elicitor families from the Dothideomycete fungal pathogensFrontiers in microbiology · 2022Article
- NOD-like receptor-mediated plant immunity: from structure to cell death.Nature reviews. Immunology · 2021Review
- A complex resistance locus in Solanum americanum recognizes a conserved Phytophthora effector.Nature plants · 2021Article
- Partial pathogenicity chromosomes in Fusarium oxysporum are sufficient to cause disease and can be horizontally transferred.Environmental microbiology · 2020Article
- An Avirulence Gene Cluster in the Wheat Stripe Rust Pathogen (Puccinia striiformis f. sp.mSphere · 2020Article
- Whole-genome sequencing of Puccinia striiformis f. sp. tritici mutant isolates identifies avirulence gene candidates.BMC genomics · 2020Article
- Mutagenesis ofFrontiers in plant science · 2020Article
- Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridisation.Nature communications · 2019Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRust fungi are an important group of plant pathogens that cause devastating losses in agricultural, silvicultural and natural ecosystems. Plants can be protected from rust disease by resistance genes encoding receptors that trigger a highly effective defence response upon recognition of specific pathogen avirulence proteins. Identifying avirulence genes is crucial for understanding how virulence evolves in the field.
resultsTo facilitate avirulence gene cloning in the flax rust fungus, Melampsora lini, we constructed a high-density genetic linkage map using single nucleotide polymorphisms detected in restriction site-associated DNA sequencing (RADseq) data. The map comprises 13,412 RADseq markers in 27 linkage groups that together span 5860 cM and contain 2756 recombination bins. The marker sequences were used to anchor 68.9 % of the M. lini genome assembly onto the genetic map. The map and anchored assembly were then used to: 1) show that M. lini has a high overall meiotic recombination rate, but recombination distribution is uneven and large coldspots exist; 2) show that substantial genome rearrangements have occurred in spontaneous loss-of-avirulence mutants; and 3) identify the AvrL2 and AvrM14 avirulence genes by map-based cloning. AvrM14 is a dual-specificity avirulence gene that encodes a predicted nudix hydrolase. AvrL2 is located in the region of the M. lini genome with the lowest recombination rate and encodes a small, highly-charged proline-rich protein.
conclusionsThe M. lini high-density linkage map has greatly advanced our understanding of virulence mechanisms in this pathogen by providing novel insights into genome variability and enabling identification of two new avirulence genes.
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