ArticleScientific reports2016
A rapid and versatile method for the isolation, purification and cryogenic storage of Schwann cells from adult rodent nerves.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 58 citations in OpenAlex.
- Culture and gene expression profiling of Schwann cells from human sural nerve.Communications biology · 2026Article
- Isolation of Brain Microglia to Study Persistence of Human Immunodeficiency Virus-1.Journal of visualized experiments : JoVE · 2026Article
- A practical CD90.2-based FACS strategy for obtaining Schwann cell-enriched cultures from adult mouse nerves.Frontiers in cellular neuroscience · 2026Article
- Limited Myelination Capacity in Human Schwann Cells in Experimental Models in Comparison to Rodent and Porcine Schwann Cells.International journal of molecular sciences · 2025Article
- Article
- Influence of Magnesium Degradation on Schwannoma Cell Responses to Nerve Injury Using an In Vitro Injury Model.Journal of functional biomaterials · 2024Article
- Article
- Human Schwann Cells in vitro I. Nerve Tissue Processing, Pre-degeneration, Isolation, and Culturing of Primary Cells.Bio-protocol · 2023Article
- Glial cell line-derived neurotrophic factor and brain-derived neurotrophic factor regulate the interaction between astrocytes and Schwann cells at the trigeminal root entry zone.Neural regeneration research · 2023Article
- Application of a Magnetic Platform in α6 Integrin-Positive iPSC-TM Purification.Bioengineering (Basel, Switzerland) · 2023Article
- Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinoma.Cell death and differentiation · 2023Article
- Models and methods to study Schwann cells.Journal of anatomy · 2022Review
- The transcriptional profile of keloidal Schwann cells.Experimental & molecular medicine · 2022Article
- An EPO-loaded multifunctional hydrogel synergizing with adipose-derived stem cells restores neurogenic erectile function via enhancing nerve regeneration and penile rehabilitation.Bioengineering & translational medicine · 2022Article
- Merlin-Deficient Schwann Cells Are More Susceptible to Radiation Injury than Normal Schwann Cells In Vitro.Journal of neurological surgery. Part B, Skull base · 2022Article
- Engineered Schwann Cell-Based Therapies for Injury Peripheral Nerve Reconstruction.Frontiers in cellular neuroscience · 2022Review
- "Tripartite Synapses" in Taste Buds: A Role for Type I Glial-like Taste Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Heregulin Activity Assays for Residual Testing of Cell Therapy Products.Biological procedures online · 2021Article
- Article
- Single-Cell Regulatory Network Inference and Clustering Identifies Cell-Type Specific Expression Pattern of Transcription Factors in Mouse Sciatic Nerve.Frontiers in cellular neuroscience · 2021Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
We herein developed a protocol for the rapid procurement of adult nerve-derived Schwann cells (SCs) that was optimized to implement an immediate enzymatic dissociation of fresh nerve tissue while maintaining high cell viability, improving yields and minimizing fibroblast and myelin contamination. This protocol introduces: (1) an efficient method for enzymatic cell release immediately after removal of the epineurium and extensive teasing of the nerve fibers; (2) an adaptable drop-plating method for selective cell attachment, removal of myelin debris, and expansion of the initial SC population in chemically defined medium; (3) a magnetic-activated cell sorting purification protocol for rapid and effective fibroblast elimination; and (4) an optional step of cryopreservation for the storage of the excess of cells. Highly proliferative SC cultures devoid of myelin and fibroblast growth were obtained within three days of nerve processing. Characterization of the initial, expanded, and cryopreserved cell products confirmed maintenance of SC identity, viability and growth rates throughout the process. Most importantly, SCs retained their sensitivity to mitogens and potential for differentiation even after cryopreservation. To conclude, this easy-to-implement and clinically relevant protocol allows for the preparation of expandable homogeneous SC cultures while minimizing time, manipulation of the cells, and exposure to culture variables.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.