ArticleJournal of the American Society for Mass Spectrometry2016
Influence of the Laser Spot Size, Focal Beam Profile, and Tissue Type on the Lipid Signals Obtained by MALDI-MS Imaging in Oversampling Mode.
Article in Journal of the American Society for Mass Spectrometry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Mass Spectrometry Imaging.Analytical chemistry · 2025Review
- Toward Omics-Scale Quantitative Mass Spectrometry Imaging of Lipids in Brain Tissue Using a Multiclass Internal Standard Mixture.Analytical chemistry · 2023Article
- A Comprehensive Comparison of Tissue Processing Methods for High-Quality MALDI Imaging of Lipids in Reconstructed Human Epidermis.Journal of the American Society for Mass Spectrometry · 2023Article
- Review
- Fate of Gold Nanoparticles in Laser Desorption/Ionization Mass Spectrometry: Toward the Imaging of Individual Nanoparticles.Journal of the American Society for Mass Spectrometry · 2023Article
- Transforming a Mid-infrared Laser Profile from Gaussian to a Top-Hat with a Diffractive Optical Element for Mass Spectrometry Imaging.Journal of the American Society for Mass Spectrometry · 2023Article
- Novel matrix strategies for improved ionization and spatial resolution using IR-MALDESI mass spectrometry imaging.Journal of mass spectrometry : JMS · 2022Article
- Spatially Resolved Mass Spectrometry at the Single Cell: Recent Innovations in Proteomics and Metabolomics.Journal of the American Society for Mass Spectrometry · 2021Review
- MALDI TOF Mass Spectrometry Imaging of Blood Smear: Method Development and Evaluation.International journal of molecular sciences · 2021Article
- Bringing SEM and MSI Closer Than Ever Before: VisualizingJournal of fungi (Basel, Switzerland) · 2020Article
- Validation of MALDI-MS imaging data of selected membrane lipids in murine brain with and without laser postionization by quantitative nano-HPLC-MS using laser microdissection.Analytical and bioanalytical chemistry · 2020Article
- Evaluation of lipid coverage and high spatial resolution MALDI-imaging capabilities of oversampling combined with laser post-ionisation.Analytical and bioanalytical chemistry · 2020Article
- Mass spectrometry imaging to detect lipid biomarkers and disease signatures in cancer.Cancer reports (Hoboken, N.J.) · 2019Review
- The Influence of MS Imaging Parameters on UV-MALDI Desorption and Ion Yield.Journal of the American Society for Mass Spectrometry · 2019Article
- Genetically Encoded Fluorescent Proteins Enable High-Throughput Assignment of Cell Cohorts Directly from MALDI-MS Images.Analytical chemistry · 2019Article
- Characterizing and alleviating ion suppression effects in atmospheric pressure matrix-assisted laser desorption/ionization.Rapid communications in mass spectrometry : RCM · 2019Article
- New insights into mechanisms of material ejection in MALDI mass spectrometry for a wide range of spot sizes.Scientific reports · 2018Article
- Matrix Optical Absorption in UV-MALDI MS.Journal of the American Society for Mass Spectrometry · 2018Article
- Five Micron High Resolution MALDI Mass Spectrometry Imaging with Simple, Interchangeable, Multi-Resolution Optical System.Journal of the American Society for Mass Spectrometry · 2017Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To improve the lateral resolution in matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) beyond the dimensions of the focal laser spot oversampling techniques are employed. However, few data are available on the effect of the laser spot size and its focal beam profile on the ion signals recorded in oversampling mode. To investigate these dependencies, we produced 2 times six spots with dimensions between ~30 and 200 μm. By optional use of a fundamental beam shaper, square flat-top and Gaussian beam profiles were compared. MALDI-MSI data were collected using a fixed pixel size of 20 μm and both pixel-by-pixel and continuous raster oversampling modes on a QSTAR mass spectrometer. Coronal mouse brain sections coated with 2,5-dihydroxybenzoic acid matrix were used as primary test systems. Sizably higher phospholipid ion signals were produced with laser spots exceeding a dimension of ~100 μm, although the same amount of material was essentially ablated from the 20 μm-wide oversampling pixel at all spot size settings. Only on white matter areas of the brain these effects were less apparent to absent. Scanning electron microscopy images showed that these findings can presumably be attributed to different matrix morphologies depending on tissue type. We propose that a transition in the material ejection mechanisms from a molecular desorption at large to ablation at smaller spot sizes and a concomitant reduction in ion yields may be responsible for the observed spot size effects. The combined results indicate a complex interplay between tissue type, matrix crystallization, and laser-derived desorption/ablation and finally analyte ionization. Graphical Abstract ᅟ.
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