ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2016
Direct matrix-assisted laser desorption ionisation time-of-flight mass spectrometry identification of mycobacteria from colonies.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.
- Application of MALDI-TOF MS to rapid identification of anaerobic bacteria.BMC infectious diseases · 2019Pooled it
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- Review and Updates on the Diagnosis of Tuberculosis.Journal of clinical medicine · 2022Review
- Routine Culture-Resistant Mycobacterium tuberculosis Rescue and Shell-Vial Assay, France.Emerging infectious diseases · 2019Article
- Differentiation ofEuropean journal of microbiology & immunology · 2019Article
- Proteotyping as alternate typing method to differentiate Campylobacter coli clades.Scientific reports · 2019Article
- Malaria, tuberculosis and HIV: what's new? Contribution of the Institut Hospitalo-Universitaire Méditerranée Infection in updated data.New microbes and new infections · 2018Article
- Automatic microscopic detection of mycobacteria in sputum: a proof-of-concept.Scientific reports · 2018Article
- Mycobacterium terramassiliense, Mycobacterium rhizamassiliense and Mycobacterium numidiamassiliense sp. nov., three new Mycobacterium simiae complex species cultured from plant roots.Scientific reports · 2018Article
- Molecular strategy for the diagnosis of infectious lymphadenitis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Article
- Recurrent bilateral Mycobacterium bovis necrotizing epididymitis: a case report.BMC research notes · 2018Article
- A protocol for culturing environmental strains of the Buruli ulcer agent, Mycobacterium ulcerans.Scientific reports · 2018Article
- Draft Genome Sequence of Mycobacterium porcinum CSURP1564.Genome announcements · 2018Article
- Buruli Ulcer: Review of a Neglected Skin Mycobacterial Disease.Journal of clinical microbiology · 2018Review
- Mycobacterium ahvazicum sp. nov., the nineteenth species of the Mycobacterium simiae complex.Scientific reports · 2018Article
- Article
- Buruli Ulcer, a Prototype for Ecosystem-Related Infection, Caused by Mycobacterium ulcerans.Clinical microbiology reviews · 2018Review
- Ready Experimental Translocation of Mycobacterium canettii Yields Pulmonary Tuberculosis.Infection and immunity · 2017Article
- Mycobacterium malmoense pulmonary infection in France: a case report.BMC research notes · 2017Article
- Mycobacterium iranicum bacteremia and hemophagocytic lymphohistiocytosis: a case report.BMC research notes · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) identification of mycobacteria requires a standard acetonitrile/formic acid pre-MALDI-TOF-MS. We prospectively compared this standard protocol with direct deposit with matrix for the identification of mycobacteria cultured on solid media. We first verified that Mycobacterium tuberculosis was killed after it was mixed with matrix. Then, 111 Mycobacterium isolates previously identified by partial rpoB gene sequencing were tested in parallel by the two protocols. An identification score >1.7 was obtained in 86/111 (77.5 %) isolates after protein extraction versus 97/111 (87.4 %) isolates after direct deposit (p = 0.039, Chi-squared test). In a third step, we determined that direct deposit achieved identification for as few as 2.10
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