ArticlePloS one2016
Molecular, Structural and Immunological Characterization of Der p 18, a Chitinase-Like House Dust Mite Allergen.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 40 citations in OpenAlex.
- Transkingdom mechanism of MAMP generation by chitotriosidase feeds oligomeric chitin from fungal pathogens and allergens into TLR2-mediated innate immune sensing.Frontiers in immunology · 2025Article
- From skin testing to molecular diagnostics: the precision leap in dust mite allergy diagnosis and clinical translation challenges.Frontiers in allergy · 2025Review
- Insect Cell-Expressed Major Ragweed Allergen Amb a 1.01 Exhibits Similar Allergenic Properties to Its Natural Counterpart from Common Ragweed Pollen.International journal of molecular sciences · 2024Article
- Immunoproteomics reveal increased serum IgG3/5 binding toFrontiers in immunology · 2023Article
- Identification of an immunodominant IgE epitope of Der p 39, a novel allergen ofThe World Allergy Organization journal · 2022Article
- Dupilumab modulates specific IgE mite responses at the molecular level in severe T2-high atopic dermatitis: A real-world experience.Frontiers in medicine · 2022Article
- House Dust Mite Precision Allergy Molecular Diagnosis (PAMD@) in the Th2-prone Atopic Dermatitis Endotype.Life (Basel, Switzerland) · 2021Article
- Der f 38 Is a Novel TLR4-Binding Allergen Related to Allergy Pathogenesis fromInternational journal of molecular sciences · 2021Article
- Analysis of Results of Specific IgE in 100 Atopic Dermatitis Patients with the Use of Multiplex Examination ALEX2-Allergy Explorer.International journal of molecular sciences · 2021Article
- Mite allergy and atopic dermatitis: Is there a clear link? (Review).Experimental and therapeutic medicine · 2020Review
- The allergenic activity and clinical impact of individual IgE-antibody binding molecules from indoor allergen sources.The World Allergy Organization journal · 2020Review
- A WAO - ARIA - GAThe World Allergy Organization journal · 2020Article
- Chitinases: Therapeutic Scaffolds for Allergy and Inflammation.Recent patents on inflammation & allergy drug discovery · 2020Review
- House Dust Mite Allergy Under Changing Environments.Allergy, asthma & immunology research · 2019Review
- Precision medicine allergy immunoassay methods for assessing immunoglobulin E sensitization to aeroallergen molecules.World journal of methodology · 2018Article
- Molecular allergen profiling in horses by microarray reveals Fag e 2 from buckwheat as a frequent sensitizer.Allergy · 2018Article
- Molecular characteristics and serodiagnostic potential of chitinase-like protein fromOncotarget · 2017Article
- Molecular aspects of allergens in atopic dermatitis.Current opinion in allergy and clinical immunology · 2017Review
- Allergens of Blomia tropicalis: An Overview of Recombinant Molecules.International archives of allergy and immunology · 2017Review
- International consensus (ICON) on: clinical consequences of mite hypersensitivity, a global problem.The World Allergy Organization journal · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
18 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe house dust mite (HDM) allergen Der p 18 belongs to the glycoside hydrolase family 18 chitinases. The relevance of Der p 18 for house dust mite allergic patients has only been partly investigated.
objectiveTo perform a detailed characterization of Der p 18 on a molecular, structural and immunological level.
methodsDer p 18 was expressed in E. coli, purified to homogeneity, tested for chitin-binding activity and its secondary structure was analyzed by circular dichroism. Der p 18-specific IgG antibodies were produced in rabbits to localize the allergen in mites using immunogold electron microscopy and to search for cross-reactive allergens in other allergen sources (i.e. mites, crustacea, mollusca and insects). IgE reactivity of rDer p 18 was tested with sera from clinically well characterized HDM-allergic patients (n = 98) and its allergenic activity was analyzed in basophil activation experiments.
resultsRecombinant Der p 18 was expressed and purified as a folded, biologically active protein. It shows weak chitin-binding activity and partial cross-reactivity with Der f 18 from D. farinae but not with proteins from the other tested allergen sources. The allergen was mainly localized in the peritrophic matrix of the HDM gut and to a lower extent in fecal pellets. Der p 18 reacted with IgE from 10% of mite allergic patients from Austria and showed allergenic activity when tested for basophil activation in Der p 18-sensitized patients.
conclusionDer p 18 is a rather genus-specific minor allergen with weak chitin-binding activity but exhibits allergenic activity and therefore should be included in diagnostic test panels for HDM allergy.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.