ArticleJournal of biomechanical engineering2016
Nonmuscle Myosin IIA Regulates Platelet Contractile Forces Through Rho Kinase and Myosin Light-Chain Kinase.
Article in Journal of biomechanical engineering, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Multiplate and TEG platelet mapping in a population of severely injured trauma patients.Transfusion medicine (Oxford, England) · 2018Trial
- Mechanotransduction of Magnetically Applied Forces Leads to Mechanical Stiffening of Platelet-Rich Plugs.Advanced healthcare materials · 2026Article
- Filamin A regulates platelet shape change and contractile force generation via phosphorylation of the myosin light chain.The Biochemical journal · 2024Article
- Megakaryocyte-induced contraction of plasma clots: cellular mechanisms and structural mechanobiology.Blood · 2024Article
- Molecular Mechanisms Linking Diabetes with Increased Risk of Thrombosis.International journal of molecular sciences · 2023Review
- Blood clot contraction: Mechanisms, pathophysiology, and disease.Research and practice in thrombosis and haemostasis · 2023Article
- Reduced platelet forces underlie impaired hemostasis in mouse models ofScience advances · 2022Article
- Review
- Microelectromechanical System Measurement of Platelet Contraction: Direct Interrogation of Myosin Light Chain Phosphorylation.International journal of molecular sciences · 2021Article
- (De)form and Function: Measuring Cellular Forces with Deformable Materials and Deformable Structures.Advanced healthcare materials · 2020Review
- Quantifying single-platelet biomechanics: An outsider's guide to biophysical methods and recent advances.Research and practice in thrombosis and haemostasis · 2020Review
- A Novel Platelet Function Assay for Trauma.The Journal of surgical research · 2020Observational
- Direct myosin-2 inhibition enhances cerebral perfusion resulting in functional improvement after ischemic stroke.Theranostics · 2020Article
- Tensile and compressive force regulation on cell mechanosensing.Biophysical reviews · 2019Review
- The biophysics and mechanics of blood from a materials perspective.Nature reviews. Materials · 2019Article
- Feeling the Force: Measurements of Platelet Contraction and Their Diagnostic Implications.Seminars in thrombosis and hemostasis · 2019Article
- Contractile forces in platelet aggregates under microfluidic shear gradients reflect platelet inhibition and bleeding risk.Nature communications · 2019Observational
- A Useful Device to Measure Kinetics of Platelet Contraction.ASAIO journal (American Society for Artificial Internal Organs : 1992)Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Platelet contractile forces play a major role in clot retraction and help to hold hemostatic clots against the vessel wall. Platelet forces are produced by its cytoskeleton, which is composed of actin and nonmuscle myosin filaments. In this work, we studied the role of Rho kinase, myosin light-chain kinase, and myosin in the generation of contractile forces by using pharmacological inhibitors and arrays of flexible microposts to measure platelet forces. When platelets were seeded onto microposts, they formed aggregates on the tips of the microposts. Forces produced by the platelets in the aggregates were measured by quantifying the deflection of the microposts, which bent in proportion to the force of the platelets. Platelets were treated with small molecule inhibitors of myosin activity: Y-27632 to inhibit the Rho kinase (ROCK), ML-7 to inhibit myosin light-chain kinase (MLCK), and blebbistatin to inhibit myosin ATPase activity. ROCK inhibition reduced platelet forces, demonstrating the importance of the assembly of actin and myosin phosphorylation in generating contractile forces. Similarly, MLCK inhibition caused weaker platelet forces, which verifies that myosin phosphorylation is needed for force generation in platelets. Platelets treated with blebbistatin also had weaker forces, which indicates that myosin's ATPase activity is necessary for platelet forces. Our studies demonstrate that myosin ATPase activity and the regulation of actin-myosin assembly by ROCK and MLCK are needed for the generation of platelet forces. Our findings illustrate and explain the importance of myosin for clot compaction in hemostasis and thrombosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.