ArticleInternational journal of molecular sciences2016
Antimicrobial Protein Candidates from the Thermophilic Geobacillus sp. Strain ZGt-1: Production, Proteomics, and Bioinformatics Analysis.
Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Purification and Identification of Antimicrobial Peptides Produced by Thermophilic Bacteria Isolated from Hot Springs.Probiotics and antimicrobial proteins · 2026Article
- Taxonomic Diversity and Antimicrobial Potential of Thermophilic Bacteria from Two Extreme Algerian Hot Springs.Microorganisms · 2025Article
- Expression of BSN314 lysozyme genes in Escherichia coli BL21: a study to demonstrate microbicidal and disintegarting potential of the cloned lysozyme.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Isolation of Thermophilic BacteriaInternational journal of dentistry · 2024Article
- Purification, Characterization and Bactericidal Action of Lysozyme, Isolated fromMolecules (Basel, Switzerland) · 2023Article
- CAMPR4: a database of natural and synthetic antimicrobial peptides.Nucleic acids research · 2023Article
- Antimicrobial Potential of the GeneraAntibiotics (Basel, Switzerland) · 2022Review
- Proteomic perspectives on thermotolerant microbes: an updated review.Molecular biology reports · 2022Review
- Isolation of a broad spectrum antimicrobial producing thermophilic Bacillus and characterization of its antimicrobial protein.Archives of microbiology · 2021Article
- Metagenomics revealing molecular profiling of community structure and metabolic pathways in natural hot springs of the Sikkim Himalaya.BMC microbiology · 2020Article
- Omics for Bioprospecting and Drug Discovery from Bacteria and Microalgae.Antibiotics (Basel, Switzerland) · 2020Review
- Collection of antimicrobial peptides database and its derivatives: Applications and beyond.Protein science : a publication of the Protein Society · 2020Article
- Towards Exploring Toxin-Antitoxin Systems inInternational journal of molecular sciences · 2019Article
- Article
- Identification of Putative Novel Class-I Lanthipeptides in Firmicutes: A CombinatorialInternational journal of molecular sciences · 2018Article
- Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A thermophilic bacterial strain, Geobacillus sp. ZGt-1, isolated from Zara hot spring in Jordan, was capable of inhibiting the growth of the thermophilic G. stearothermophilus and the mesophilic Bacillus subtilis and Salmonella typhimurium on a solid cultivation medium. Antibacterial activity was not observed when ZGt-1 was cultivated in a liquid medium; however, immobilization of the cells in agar beads that were subjected to sequential batch cultivation in the liquid medium at 60 °C showed increasing antibacterial activity up to 14 cycles. The antibacterial activity was lost on protease treatment of the culture supernatant. Concentration of the protein fraction by ammonium sulphate precipitation followed by denaturing polyacrylamide gel electrophoresis separation and analysis of the gel for antibacterial activity against G. stearothermophilus showed a distinct inhibition zone in 15-20 kDa range, suggesting that the active molecule(s) are resistant to denaturation by SDS. Mass spectrometric analysis of the protein bands around the active region resulted in identification of 22 proteins with molecular weight in the range of interest, three of which were new and are here proposed as potential antimicrobial protein candidates by in silico analysis of their amino acid sequences. Mass spectrometric analysis also indicated the presence of partial sequences of antimicrobial enzymes, amidase, and dd-carboxypeptidase.
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