Evidence map›Paper›PMID 27547441›Full record

ArticleLupus science & medicine2016

Assessment of direct versus indirect magnetic bead-based T-cell isolation procedures followed by magnetic bead-based DNA isolation.

Anna Rosenbaum, Ellen Bleck, Matthias Schneider, Georg Pongratz, Stefan Vordenbäumen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Lupus science & medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Anna RosenbaumMedical Faculty , Hiller Research Center Rheumatology at University Hospital Düsseldorf, Heinrich-Heine-University , Düsseldorf , Germany.
Ellen BleckMedical Faculty , Hiller Research Center Rheumatology at University Hospital Düsseldorf, Heinrich-Heine-University , Düsseldorf , Germany.
Matthias SchneiderMedical Faculty , Hiller Research Center Rheumatology at University Hospital Düsseldorf, Heinrich-Heine-University , Düsseldorf , Germany.
Georg PongratzMedical Faculty , Hiller Research Center Rheumatology at University Hospital Düsseldorf, Heinrich-Heine-University , Düsseldorf , Germany.
Stefan VordenbäumenMedical Faculty , Hiller Research Center Rheumatology at University Hospital Düsseldorf, Heinrich-Heine-University , Düsseldorf , Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo compare direct and indirect bead-based T-cell isolation followed by magnetic bead-based DNA isolation.

methodsT-cells were isolated by direct or indirect selection with magnetic bead coated antbiodies followed by magnetic bead-based automated DNA isolation in 10 healthy subjects. Purity of T-cells, purity of DNA (by A260/A280 ratio measurement) and DNA concentration were assessed.

resultsDirect and indirect labelling resulted in comparable T-cell purity (93.11±1.47% vs. 94.99±1.54%, p= 0.125) and DNA concentration per cell (50.97±14.15 ng/(mlxcell) vs. 49.53±13.62 ng/(mlxcell), p=0.492), while DNA purity was significantly higher after direct labelling (1.82±0.05 vs. 1.78±0.03, p=0.0488).

conclusionsBoth direct and indirect magnetic bead-based T-cell selection may be used prior to magnetic bead-based DNA isolation procedures.

Indexed as

DNA isolationepigeneticsmagnetic beadsT Cells

Identifiers

PMID27547441
PMCPMC4964240

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.