ArticleCell reports2016
Synthetic Biology Platform for Sensing and Integrating Endogenous Transcriptional Inputs in Mammalian Cells.
Article in Cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 51 citations in OpenAlex.
- Article
- Extending dynamic and operational range of the biosensor responding to l-carnitine by directed evolution.Synthetic and systems biotechnology · 2025Article
- Engineering Microbial Consortia as Living Materials: Advances and Prospectives.ACS synthetic biology · 2024Review
- A noncommutative combinatorial protein logic circuit controls cell orientation in nanoenvironments.Science advances · 2023Article
- Synthetic gene circuits for cell state detection and protein tuning in human pluripotent stem cells.Molecular systems biology · 2022Article
- Engineering digitizer circuits for chemical and genetic screens in human cells.Nature communications · 2021Article
- Model-guided design of mammalian genetic programs.Science advances · 2021Article
- Rational gRNA design based on transcription factor binding data.Synthetic biology (Oxford, England) · 2021Article
- A platform for post-translational spatiotemporal control of cellular proteins.Synthetic biology (Oxford, England) · 2021Article
- Precise determination of input-output mapping for multimodal gene circuits using data from transient transfection.PLoS computational biology · 2020Article
- Engineered systems of inducible anti-repressors for the next generation of biological programming.Nature communications · 2020Article
- De novo design of protein logic gates.Science (New York, N.Y.) · 2020Article
- The COMET toolkit for composing customizable genetic programs in mammalian cells.Nature communications · 2020Article
- Artificial signaling in mammalian cells enabled by prokaryotic two-component system.Nature chemical biology · 2020Article
- Oncolytic adenovirus programmed by synthetic gene circuit for cancer immunotherapy.Nature communications · 2019Article
- Biofunctionalized Materials Featuring Feedforward and Feedback Circuits Exemplified by the Detection of Botulinum Toxin A.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
- Biomolecular Assemblies: Moving from Observation to Predictive Design.Chemical reviews · 2018Review
- Sigma Factor-Mediated Tuning of Bacterial Cell-Free Synthetic Genetic Oscillators.ACS synthetic biology · 2018Article
- Programmable protein circuits in living cells.Science (New York, N.Y.) · 2018Article
- Programming Morphogenesis through Systems and Synthetic Biology.Trends in biotechnology · 2018Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One of the goals of synthetic biology is to develop programmable artificial gene networks that can transduce multiple endogenous molecular cues to precisely control cell behavior. Realizing this vision requires interfacing natural molecular inputs with synthetic components that generate functional molecular outputs. Interfacing synthetic circuits with endogenous mammalian transcription factors has been particularly difficult. Here, we describe a systematic approach that enables integration and transduction of multiple mammalian transcription factor inputs by a synthetic network. The approach is facilitated by a proportional amplifier sensor based on synergistic positive autoregulation. The circuits efficiently transduce endogenous transcription factor levels into RNAi, transcriptional transactivation, and site-specific recombination. They also enable AND logic between pairs of arbitrary transcription factors. The results establish a framework for developing synthetic gene networks that interface with cellular processes through transcriptional regulators.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.