ArticleNature communications2016
Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 85 citations in OpenAlex.
- Viscoelastic characterization of the lipid cubic phase provides insights into high-viscosity extrusion injection for XFEL experiments.Scientific reports · 2025Article
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- Crystal structure via fluctuation scattering.IUCrJ · 2024Article
- Energetics of the H-Bond Network inBiochemistry · 2024Article
- The time revolution in macromolecular crystallography.Structural dynamics (Melville, N.Y.) · 2024Article
- From femtoseconds to minutes: time-resolved macromolecular crystallography at XFELs and synchrotrons.Acta crystallographica. Section D, Structural biology · 2024Article
- Advanced manufacturing provides tailor-made solutions for crystallography with x-ray free-electron lasers.Structural dynamics (Melville, N.Y.) · 2024Article
- A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers.Nature communications · 2023Article
- Review of serial femtosecond crystallography including the COVID-19 pandemic impact and future outlook.Structure (London, England : 1993) · 2023Review
- A versatile approach to high-density microcrystals in lipidic cubic phase for room-temperature serial crystallography.Journal of applied crystallography · 2023Article
- Correction of rhodopsin serial crystallography diffraction intensities for a lattice-translocation defect.Acta crystallographica. Section D, Structural biology · 2023Article
- Observations of phase changes in monoolein during high viscous injection.Journal of synchrotron radiation · 2022Article
- 3D printed devices and infrastructure for liquid sample delivery at the European XFEL.Journal of synchrotron radiation · 2022Article
- Crystal structure of CmABCB1 multi-drug exporter in lipidic mesophase revealed by LCP-SFX.IUCrJ · 2022Article
- Dynamic Structural Biology Experiments at XFEL or Synchrotron Sources.Methods in molecular biology (Clifton, N.J.) · 2021Review
- High-throughput in situ experimental phasing.Acta crystallographica. Section D, Structural biology · 2020Article
- Photoswitching mechanism of a fluorescent protein revealed by time-resolved crystallography and transient absorption spectroscopy.Nature communications · 2020Article
- A fixed-target platform for serial femtosecond crystallography in a hydrated environment.IUCrJ · 2020Article
- The complementarity of serial femtosecond crystallography and MicroED for structure determination from microcrystals.Current opinion in structural biology · 2019Review
Corrections and comments
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Authors and funding
40 authors at 9 institutions in 5 countries.
Funding
Abstract
Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 Å resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1 ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1 mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.