ArticleNeuron2016
High-Throughput Mapping of Single-Neuron Projections by Sequencing of Barcoded RNA.
Article in Neuron, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 217 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
217 citing papers in PubMed, 1 synthesis or guideline pooled it, 401 citations in OpenAlex.
- Reference-based cell type matching of in situ image-based spatial transcriptomics data on primary visual cortex of mouse brain.Scientific reports · 2023Pooled it
- POINTseq: Cell-type-specific barcoding reveals single-cell projection architecture of the mouse dopaminergic system.Neuron · 2026Article
- Mapping brain-wide monosynaptic inputs to single neurons with ROInet-seq.Cell reports methods · 2026Article
- Article
- Barcoded Rabies In Situ Connectomics for high-throughput reconstruction of neural circuits.Nature communications · 2026Article
- Brain-first and body-first subtypes of Lewy body disease.Nature reviews. Neurology · 2026Review
- Conserved and specialized features of thalamocortical wiring revealed by single-cell projection mapping in mouse and marmoset.bioRxiv : the preprint server for biology · 2026Article
- Article
- Complementary influences of noradrenaline and dopamine in orchestrating a motivated social behavior.Current opinion in neurobiology · 2026Review
- Visual field position shapes input sampling and output routing in the superior colliculus.bioRxiv : the preprint server for biology · 2026Article
- BARseq3: a modular system for integrating spatial multi-omics and cellular barcoding in single cells.bioRxiv : the preprint server for biology · 2026Article
- Spatiotemporally dynamic noradrenergic regulation of cortical networks.bioRxiv : the preprint server for biology · 2026Article
- A Guide for Spatial Omics Technologies: Innovation, Evaluation, and Application.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Inferring norepinephrine dynamics from partial observations reveals the temporal structure of elevations during arousal.bioRxiv : the preprint server for biology · 2026Article
- Humanized extracellular vesicles for efficient RNA delivery.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Cell type-specific barcoding reveals the single-neuron projectional architecture of the mouse midbrain dopaminergic system.bioRxiv : the preprint server for biology · 2026Article
- MAPseq2: a sensitive barcoded connectomics method.bioRxiv : the preprint server for biology · 2026Article
- Connectome-seq: high-throughput mapping of neuronal connectivity at single-synapse resolution via barcode sequencing.Nature methods · 2026Article
- Synaptic connectomics by sequencing.Nature methods · 2026Article
- The role of the locus coeruleus in eye movements during perceptual decision making.bioRxiv : the preprint server for biology · 2026Article
157 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Neurons transmit information to distant brain regions via long-range axonal projections. In the mouse, area-to-area connections have only been systematically mapped using bulk labeling techniques, which obscure the diverse projections of intermingled single neurons. Here we describe MAPseq (Multiplexed Analysis of Projections by Sequencing), a technique that can map the projections of thousands or even millions of single neurons by labeling large sets of neurons with random RNA sequences ("barcodes"). Axons are filled with barcode mRNA, each putative projection area is dissected, and the barcode mRNA is extracted and sequenced. Applying MAPseq to the locus coeruleus (LC), we find that individual LC neurons have preferred cortical targets. By recasting neuroanatomy, which is traditionally viewed as a problem of microscopy, as a problem of sequencing, MAPseq harnesses advances in sequencing technology to permit high-throughput interrogation of brain circuits.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.