Evidence map›Paper›PMID 27544307›Full record

ArticleExperimental eye research2016

Flow cytometric analysis of inflammatory and resident myeloid populations in mouse ocular inflammatory models.

Sidath E Liyanage, Peter J Gardner, Joana Ribeiro, Enrico Cristante, Robert D Sampson, Ulrich F O Luhmann, Robin R Ali, James W Bainbridge

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental eye research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Sidath E LiyanageUCL Institute of Ophthalmology, 11-43 Bath Street, London, EC1V 9EL, UK.
Peter J GardnerUCL Institute of Ophthalmology, 11-43 Bath Street, London, EC1V 9EL, UK.
Joana RibeiroUCL Institute of Ophthalmology, 11-43 Bath Street, London, EC1V 9EL, UK.
Enrico CristanteUCL Institute of Ophthalmology, 11-43 Bath Street, London, EC1V 9EL, UK.
Robert D SampsonUCL Institute of Ophthalmology, 11-43 Bath Street, London, EC1V 9EL, UK.
Ulrich F O LuhmannUCL Institute of Ophthalmology, 11-43 Bath Street, London, EC1V 9EL, UK.
Robin R AliUCL Institute of Ophthalmology, 11-43 Bath Street, London, EC1V 9EL, UK; NIHR Biomedical Research Centre for Ophthalmology, Moorfields Eye Hospital and UCL Institute of Ophthalmology, City Road, London, EC1V 2PD, UK.
James W BainbridgeUCL Institute of Ophthalmology, 11-43 Bath Street, London, EC1V 9EL, UK; NIHR Biomedical Research Centre for Ophthalmology, Moorfields Eye Hospital and UCL Institute of Ophthalmology, City Road, London, EC1V 2PD, UK.

Funding

Department of Health NIHR-RP-011-003Medical Research Council MR/K003003/1Medical Research Council MR/L012758/1Medical Research Council MR/L022699/1
6 · The paper itself

Abstract

Myeloid cells make a pivotal contribution to tissue homeostasis during inflammation. Both tissue-specific resident populations and infiltrating myeloid cells can cause tissue injury through aberrant activation and/or dysregulated activity. Reliable identification and quantification of myeloid cells within diseased tissues is important to understand pathological inflammatory processes. Flow cytometry is a valuable technique for leukocyte analysis, but a standardized flow cytometric method for myeloid cell populations in the eye is lacking. Here, we validate a reproducible flow cytometry gating approach to characterize myeloid cells in several commonly used models of ocular inflammation. We profile and quantify myeloid subsets across these models, and highlight the value of this strategy in identifying phenotypic differences using Ccr2-deficient mice. This method will aid standardization in the field and facilitate future investigations into the roles of myeloid cells during ocular inflammation.

Indexed as

AnimalsAutoimmune DiseasesCell CountDisease Models, AnimalFlow CytometryMiceMice, Inbred C57BLMyeloid CellsRetinal Pigment EpitheliumRetinitisUveitisChoroidal neovascularizationEndotoxin-induced uveitisExperimental autoimmune uveoretinitisFlow cytometryMonocytesMyeloid cellsNeutrophilsRetinal microglia

Identifiers

PMID27544307
PMCPMC5053376

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.