Evidence map›Paper›PMID 27544228›Full record

ArticleJournal of physiology and biochemistry2016

Leucine supplementation at the onset of high-fat feeding does not prevent weight gain or improve glycemic regulation in male Sprague-Dawley rats.

Jamie I Baum, Tyrone A Washington, Stephanie A Shouse, Walter Bottje, Sami Dridi, Gina Davis, Dameon Smith

Abstract read
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In one paragraph

Article in Journal of physiology and biochemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Jamie I BaumDepartment of Food Science, University of Arkansas, 2650 North Young Avenue, Fayetteville, AR, 72704, USA. baum@uark.edu.
Tyrone A WashingtonDepartment of Health, Human Performance and Recreation, University of Arkansas, Fayetteville, AR, 72701, USA.
Stephanie A ShouseDepartment of Food Science, University of Arkansas, 2650 North Young Avenue, Fayetteville, AR, 72704, USA.
Walter BottjeDepartment of Poultry Science, Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR, 72701, USA.
Sami DridiDepartment of Poultry Science, Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR, 72701, USA.
Gina DavisDepartment of Health, Human Performance and Recreation, University of Arkansas, Fayetteville, AR, 72701, USA.
Dameon SmithDepartment of Food Science, University of Arkansas, 2650 North Young Avenue, Fayetteville, AR, 72704, USA.
University of Arkansas at Fayetteville · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Obesity is a major public health concern and it is essential to identify effective treatments and preventative strategies to stop continued increases in obesity rates. The potential functional roles of the branched chain amino acid leucine make this amino acid an attractive candidate for the treatment and/or prevention of obesity. The objective of this study was to determine if long-term leucine supplementation could prevent the development of obesity and reduce the risk factors for chronic disease in rats fed a high-fat (60 % fat) diet. Male Sprague-Dawley rats (n = 30 per dietary treatment) were meal-fed (3 meals/day) either a control, low-fat diet (LF), control + leucine (LFL), high-fat (HF), or high-fat + leucine (HFL) for 42 days. On day 42, rats were sacrificed at 0, 30, or 90 min postprandial. Animals fed the HF and HFL diets had higher (P < 0.05) final body weights and weight gain compared to animals fed the LF and LFL diets. Leucine supplementation increased epididymal fat mass (P < 0.05) and decreased muscle mass (P < 0.05). There was no effect of leucine supplementation on postprandial glucose or insulin response. However, there was a significant effect (P < 0.05) of diet and time on free fatty acid concentrations. There was no effect of leucine on muscle markers of protein synthesis (4E-BP1, p70S6K) or energy metabolism (Akt, AMPK). Leucine supplementation decreased (P < 0.05) PGC1α expression and increased (P < 0.05) PPARγ expression in skeletal muscle. In conclusion, long-term leucine supplementation does not prevent weight gain, improve body composition, or improve glycemic control in rats fed a high-fat diet.

Indexed as

Dietary SupplementsAdipose TissueAMP-Activated Protein KinasesAnimalsBiomarkersBlood GlucoseBody CompositionCarrier ProteinsDietary FatsDiet, High-FatEnergy MetabolismFatty Acids, NonesterifiedGene ExpressionInsulinIntracellular Signaling Peptides and ProteinsLeucineAMP-Activated Protein KinasesBiomarkersBlood GlucoseCarrier ProteinsDietary FatsEif4ebp1 protein, ratFatty Acids, NonesterifiedInsulinIntracellular Signaling Peptides and ProteinsLeucinePeroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alphaPhosphoproteinsPPAR gammaPpargc1a protein, ratProto-Oncogene Proteins c-aktRibosomal Protein S6 Kinases, 70-kDaAmino acidsBody compositionGlycemic responseLeucineObesitySkeletal muscle

Identifiers

PMID27544228
OpenAlexW2509492815

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.