Evidence map›Paper›PMID 27542266›Full record

ArticleOncotarget2016

DCUN1D3 activates SCFSKP2 ubiquitin E3 ligase activity and cell cycle progression under UV damage.

Shuai Zhang, Jing Huang, Taiping Shi, Fanlei Hu, Li Zhang, Ping-Kun Zhou, Dalong Ma, Teng Ma, Xiaoyan Qiu

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oncotarget, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Ubiquitin Proteasome Gene Signatures in Ependymoma Molecular Subtypes.International journal of molecular sciences · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Shuai ZhangDepartment of Immunology, School of Basic Medical Sciences, Peking University, Beijing, China.
Jing HuangDepartment of Immunology, School of Basic Medical Sciences, Peking University, Beijing, China.
Taiping ShiDepartment of Immunology, School of Basic Medical Sciences, Peking University, Beijing, China.
Fanlei HuDepartment of Immunology, School of Basic Medical Sciences, Peking University, Beijing, China.
Li ZhangDepartment of Immunology, School of Basic Medical Sciences, Peking University, Beijing, China.
Ping-Kun ZhouDepartment of Radiation Toxicology and Oncology, Beijing Key Laboratory for Radiobiology (BKLRB), Beijing Institute of Radiation Medicine, Beijing, P. R. China.
Dalong MaDepartment of Immunology, School of Basic Medical Sciences, Peking University, Beijing, China.
Teng MaDepartment of Immunology, School of Basic Medical Sciences, Peking University, Beijing, China.
Xiaoyan QiuDepartment of Immunology, School of Basic Medical Sciences, Peking University, Beijing, China.
Peking University · CNBeijing Radiation Center · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Our previous study showed that knockdown the endogenous expression of DCUN1D3 (also called SCCRO3 or DCNL3) blocked the S phase progression after UV irradiation. Here, we show that the silence of DCUN1D3 can increase the cyclin-dependent kinase inhibitor p27 protein levels after UV irradiation. Through Co-immunoprecipitation experiments, we found that DCUN1D3 bound to CAND1. And DCUN1D3 knockdown synergized with CAND1 over-expression in arresting the S phase. Given the CAND1's established role in Cullin-1 neddylation, we found Cullin-1 was less neddylated in DCUN1D3 deficient cells. So the silence of DCUN1D3 can inhibit the formation of SCFSKP2 complex by reducing Cullin-1 neddylation. Given that p27 is the primary target of SCFSKP2 complex, the cells lost DCUN1D3 showed a remarkable accumulation of p27 to cause S phase block.

Indexed as

Carrier ProteinsCell CycleCell Cycle ProteinsCell Line, TumorCyclin-Dependent Kinase Inhibitor p27Gene Expression Regulation, NeoplasticGene SilencingGlutathione TransferaseHeLa CellsHumansProtein BindingSKP Cullin F-Box Protein LigasesS PhaseS-Phase Kinase-Associated ProteinsTranscription FactorsUbiquitin-Protein LigasesCAND1 protein, humanCarrier ProteinsCDKN1B protein, humanCell Cycle ProteinsCyclin-Dependent Kinase Inhibitor p27DCUN1D3 protein, humanGlutathione TransferaseRBX1 protein, humanSKP Cullin F-Box Protein LigasesS-Phase Kinase-Associated ProteinsTranscription FactorsUbiquitin-Protein LigasesCAND1cell cycleDCUN1D3p27SCFSKP2

Identifiers

PMID27542266
PMCPMC5295445
OpenAlexW2507615627

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.