ArticleeLife2016
Physical determinants of vesicle mobility and supply at a central synapse.
Article in eLife, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 54 citations in OpenAlex.
- Independently engaging protein tethers of different length enhance synaptic vesicle trafficking to the plasma membrane.The Journal of physiology · 2025Article
- Cellular and synaptic specializations for sub-millisecond precision in the mammalian auditory brainstem.Frontiers in cellular neuroscience · 2025Review
- Single-vesicle imaging reveals actin-dependent spatial restriction of vesicles at the active zone, essential for sustained transmission.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Real-Time Tracking of Vesicles in Living Cells Reveals That Tau-Hyperphosphorylation Suppresses Unidirectional Transport by Motor Proteins.Chemical & biomedical imaging · 2024Article
- Vesicle and reaction-diffusion hybrid modeling with STEPS.Communications biology · 2024Article
- Parallel processing of quickly and slowly mobilized reserve vesicles in hippocampal synapses.eLife · 2024Article
- Quantitative analysis of autophagy reveals the role of ATG9 and ATG2 in autophagosome formation.The Journal of cell biology · 2023Article
- Microtubule depolymerization contributes to spontaneous neurotransmitter release in vitro.Communications biology · 2023Article
- Article
- Validation of a stereological method for estimating particle size and density from 2D projections with high accuracy.PloS one · 2023Article
- Coarse-Grained Simulations of Release of Drugs Housed in Flexible Nanogels: New Insights into Kinetic Parameters.Polymers · 2022Article
- Endosomal phosphatidylinositol 3-phosphate controls synaptic vesicle cycling and neurotransmission.The EMBO journal · 2022Article
- Article
- Axon terminals control endolysosome diffusion to support synaptic remodelling.Life science alliance · 2021Article
- Article
- Calcium-vesicles perform active diffusion in the sea urchin embryo during larval biomineralization.PLoS computational biology · 2021Article
- The frequency-dependent effect of electrical fields on the mobility of intracellular vesicles in astrocytes.Biochemical and biophysical research communications · 2021Article
- Quantitative Synaptic Biology: A Perspective on Techniques, Numbers and Expectations.International journal of molecular sciences · 2020Review
- A comparative analysis of the mobility of 45 proteins in the synaptic bouton.The EMBO journal · 2020Article
- Direct imaging of rapid tethering of synaptic vesicles accompanying exocytosis at a fast central synapse.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 4 countries.
Funding
Abstract
Encoding continuous sensory variables requires sustained synaptic signalling. At several sensory synapses, rapid vesicle supply is achieved via highly mobile vesicles and specialized ribbon structures, but how this is achieved at central synapses without ribbons is unclear. Here we examine vesicle mobility at excitatory cerebellar mossy fibre synapses which sustain transmission over a broad frequency bandwidth. Fluorescent recovery after photobleaching in slices from VGLUT1(Venus) knock-in mice reveal 75% of VGLUT1-containing vesicles have a high mobility, comparable to that at ribbon synapses. Experimentally constrained models establish hydrodynamic interactions and vesicle collisions are major determinants of vesicle mobility in crowded presynaptic terminals. Moreover, models incorporating 3D reconstructions of vesicle clouds near active zones (AZs) predict the measured releasable pool size and replenishment rate from the reserve pool. They also show that while vesicle reloading at AZs is not diffusion-limited at the onset of release, diffusion limits vesicle reloading during sustained high-frequency signalling.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.