ArticleCellular and molecular neurobiology2017
Long-Term Consumption of High-Fat Diet in Rats: Effects on Microglial and Astrocytic Morphology and Neuronal Nitric Oxide Synthase Expression.
Article in Cellular and molecular neurobiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 50 citations in OpenAlex.
- Treadmill exercise impedes cognitive decline in type 2 diabetic rats by improving mitochondrial function and modulating the TGF-β/Smad-3 pathway.Pflugers Archiv : European journal of physiology · 2026Article
- Reduction of Dietary Fat Rescues High-Fat Diet-Induced Depressive Phenotypes and the Associated Hippocampal Astrocytic Deficits in Mice.Metabolites · 2025Article
- Alterations of Purinergic Receptors Levels and Their Involvement in the Glial Cell Morphology in a Pre-Clinical Model of Autism Spectrum Disorders.Brain sciences · 2023Article
- Article
- Brainstem astrocytes control homeostatic regulation of caloric intake.The Journal of physiology · 2023Article
- Obesity-induced neuroinflammation and cognitive impairment in young adult versus middle-aged mice.Immunity & ageing : I & A · 2022Article
- Sodium-glucose cotransporter 2 inhibitor ameliorates high fat diet-induced hypothalamic-pituitary-ovarian axis disorders.The Journal of physiology · 2022Review
- Neuroinflammatory responses following zinc or branched-chain amino acids supplementation in obese rats.Metabolic brain disease · 2022Article
- Sex differences in the fecal microbiome and hippocampal glial morphology following diet and antibiotic treatment.PloS one · 2022Article
- Short high fat diet triggers reversible and region specific effects in DCXScientific reports · 2021Article
- Astroglia in Autism Spectrum Disorder.International journal of molecular sciences · 2021Review
- Chronic Stress Alters Astrocyte Morphology in Mouse Prefrontal Cortex.The international journal of neuropsychopharmacology · 2021Article
- Review
- Mapping of Microglial Brain Region, Sex and Age Heterogeneity in Obesity.International journal of molecular sciences · 2021Review
- MRI spectroscopic and tractography studies indicate consequences of long-term ketogenic diet.Brain structure & function · 2020Article
- A Western diet impairs CNS energy homeostasis and recovery after spinal cord injury: Link to astrocyte metabolism.Neurobiology of disease · 2020Article
- Brain Metabolism Alterations in Type 2 Diabetes: What Did We Learn From Diet-Induced Diabetes Models?Frontiers in neuroscience · 2020Review
- Tipping the scales: Are females more at risk for obesity- and high-fat diet-induced hypertension and vascular dysfunction?British journal of pharmacology · 2019Review
- TLR4 inhibitor TAK-242 attenuates the adverse neural effects of diet-induced obesity.Journal of neuroinflammation · 2018Article
- Conditioned Medium of Human Adipose Mesenchymal Stem Cells Increases Wound Closure and Protects Human Astrocytes Following Scratch Assay In Vitro.Molecular neurobiology · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Obesity in humans is associated with cognitive decline and elevated risk of neurodegenerative diseases of old age. Variations of high-fat diet are often used to model these effects in animal studies. However, we previously reported improvements in markers of memory and learning, as well as larger hippocampi and higher metabolite concentrations in Wistar rats fed high-fat, high-carbohydrate diet (HFCD, 60 % energy from fat, 28 % from carbohydrates) for 1 year; this diet leads to mild ketonemia (Setkowicz et al. in PLoS One 10:e0139987, 2015). In the present study, we follow up on this cohort to assess glial morphology and expression of markers related to gliosis. Twenty-five male Wistar rats were kept on HFCD and twenty-five on normal chow. At 12 months of age, the animals were sacrificed and processed for immunohistochemical staining for astrocytic (glial fibrillary acidic protein), microglial (Iba1), and neuronal (neuronal nitric oxide synthetase, nNOS) markers in the hippocampus. We have found changes in immunopositive area fraction and cellular complexity, as studied by a simplified Sholl procedure. To our knowledge, this study is the first to apply this methodology to the study of glial cells in HFCD animals. GFAP and Iba1 immunoreactive area fraction in the hippocampi of HFCD-fed rats were decreased, while the mean number of intersections (an indirect measure of cell complexity) was decreased in GFAP-positive astrocytes, but not in Iba1-expressing microglia. At the same time, nNOS expression was lowered after HFCD in both the cortex and the hippocampus.
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