Evidence map›Paper›PMID 27540975›Full record

ArticleCancer biomarkers : section A of Disease markers2016

Silencing of UCA1, a poor prognostic factor, inhibited the migration of endometrial cancer cell.

Lin Lu, Yuan Shen, Kuo-Fu Tseng, Wenlian Liu, Hui Duan, Wei Meng

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PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer biomarkers : section A of Disease markers, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed, 3 pooled it
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 3 syntheses or guidelines pooled it, 32 citations in OpenAlex.

  1. Pooled it
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  10. Non-Coding RNAs in Endometrial Physiopathology.International journal of molecular sciences · 2018
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Lin LuGynecology and Obstetrics Department, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
Yuan ShenGynecology and Obstetrics Department, the First Affiliated Hospital of Jinan University, Guangzhou, Guangdong, China.
Kuo-Fu TsengBiophysics Department, Oregon State University, Corvallis, OR, USA.
Wenlian LiuGynecology and Obstetrics Department, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
Hui DuanGynecology and Obstetrics Department, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
Wei MengInstitute of Genetic Engineering, Southern Medical University, Guangzhou, Guangdong, China.
Southern Medical University · CNOregon State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundEndometrial cancer (EC) is a common female malignancy. Most patients were diagnosed at an early stage with a favorable prognosis. But more than 30% patients were high risk at III/IV stage with invading deep into the myometrium and progressively lead to local or extra pelvic metastasis. Urothelial cancer-associated 1 (UCA1) had been reported as the oncogenic long non-coding RNA in many tumors, but the role of UCA1 in EC is still unclear.

methodQRT-PCR was used to analysis the level of UCA1 in the proliferative endometrium, primary EC tissue and lymph node metastasis tissue of EC. According to the QRT-PCR results of primary EC tissue, survival curves were made using the Kaplan-Meier method, and the log rank test was used to analyze the differences of clinicopathological characteristics and survival between the low expression and high expression group of UCA1 in EC patients. Moreover we knocked down the expression of UCA1 in EC cell lines HTB-111 and Ishikawa, and detected the migration and invasion ability of them with wound healing assay and transwell assay.

resultsThe expression level of UCA1 using QRT-PCR method in lymph node metastasis tissue was the highest than that in the proliferative endometrium and primary EC tissues (1.15 ± 0.23, 3.23 ± 1.06 vs. 6.42 ± 1.46, P < 0.0001). For the primary EC tissue, the median fold change of UCA1 was used as a cutoff value. High UCA1 expression was observed to be closely correlated with lymph node metastasis (P = 0.008), distant metastasis (P = 0.003), grade (P = 0.009), advanced TNM stage (P = 0.031) and vessel invasive (P = 0.032). The 5-year overall survival rate in the high expression group was 41.7%, compared with 72.7% in the low expression group (P = 0.023). After silencing the UCA1, the migration and invasion ability of EC cell lines reduced significantly.

conclusionOur findings provide the convincing evidence that the UCA1 plays an important role in the metastasis of EC and may serve as a novel molecular marker to predict the aggressive tumor progression and unfavorable prognosis of EC patients.

Indexed as

Gene SilencingAdultAgedBiomarkers, TumorCell Line, TumorCell MovementEndometrial NeoplasmsFemaleHumansKaplan-Meier EstimateMiddle AgedNeoplasm GradingNeoplasm MetastasisNeoplasm StagingPrognosisRNA, Long NoncodingBiomarkers, TumorRNA, Long NoncodingUCA1 RNA, humanEndometrial cancerLong noncoding RNAmetastasisUCA1

Identifiers

PMID27540975
PMCPMC13020495
OpenAlexW2466406798

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.