ArticleAutophagy2016
Comparative analyses of ubiquitin-like ATG8 and cysteine protease ATG4 autophagy genes in the plant lineage and cross-kingdom processing of ATG8 by ATG4.
Article in Autophagy, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 67 citations in OpenAlex.
- ATG4B-dependent processing of tandem-domain BmLC3 exposes a Tyr136-regulated C-terminal fragment.Frontiers in cell and developmental biology · 2026Article
- Evaluating the Antifungal Potential of Autophagy-Related Protein 4 (ATG4) Inhibitors against Human Fungal Pathogens.Journal of microbiology and biotechnology · 2025Article
- An A. thaliana mutant lacking all nine ATG8 isoforms provides genetic evidence for functional specialization of ATG8 in plants.Journal of cell science · 2025Article
- Autophagy restricts tomato fruit ripening via a general role in ethylene repression.The New phytologist · 2025Article
- Clathrin light chains negatively regulate plant immunity by hijacking the autophagy pathway.Plant communications · 2024Article
- The Role of Plant Ubiquitin-like Modifiers in the Formation of Salt Stress Tolerance.Plants (Basel, Switzerland) · 2024Review
- The Phytophthora parasitica effector AVH195 interacts with ATG8, attenuates host autophagy, and promotes biotrophic infection.BMC biology · 2024Article
- Autophagy formation, microtubule disorientation, and alteration of ATG8 and tubulin gene expression under simulated microgravity in Arabidopsis thaliana.NPJ microgravity · 2024Article
- Systematic analysis and expression of Gossypium ATG8 family reveals the roles of GhATG8f responding to salt stress in cotton.Plant cell reports · 2024Article
- Atg8 family proteins, LIR/AIM motifs and other interaction modes.Autophagy reports · 2023Article
- Mechanisms controlling plant proteases and their substrates.Cell death and differentiation · 2023Review
- Role of autophagy-related proteins ATG8f and ATG8h in the maintenance of autophagic activity inFrontiers in plant science · 2023Article
- Evolutionary diversification of the autophagy-related ubiquitin-like conjugation systems.Autophagy · 2022Article
- Genome-Wide Identification and Functional Characterization Reveals the Pivotal Roles ofInternational journal of molecular sciences · 2022Article
- Autophagy modulates the metabolism and growth of tomato fruit during development.Horticulture research · 2022Article
- Autophagy modulates growth and development in the mossFrontiers in plant science · 2022Article
- Autophagy Is Involved in the Viability of Overexpressing ThioredoxinAntioxidants (Basel, Switzerland) · 2021Article
- P300/HDAC1 regulates the acetylation/deacetylation and autophagic activities of LC3/Atg8-PE ubiquitin-like system.Cell death discovery · 2021Article
- Autophagy in Plant Abiotic Stress Management.International journal of molecular sciences · 2021Review
- Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autophagy is important for degradation and recycling of intracellular components. In a diversity of genera and species, orthologs and paralogs of the yeast Atg4 and Atg8 proteins are crucial in the biogenesis of double-membrane autophagosomes that carry the cellular cargoes to vacuoles and lysosomes. Although many plant genome sequences are available, the ATG4 and ATG8 sequence analysis is limited to some model plants. We identified 28 ATG4 and 116 ATG8 genes from the available 18 different plant genome sequences. Gene structures and protein domain sequences of ATG4 and ATG8 are conserved in plant lineages. Phylogenetic analyses classified ATG8s into 3 subgroups suggesting divergence from the common ancestor. The ATG8 expansion in plants might be attributed to whole genome duplication, segmental and dispersed duplication, and purifying selection. Our results revealed that the yeast Atg4 processes Arabidopsis ATG8 but not human LC3A (HsLC3A). In contrast, HsATG4B can process yeast and plant ATG8s in vitro but yeast and plant ATG4s cannot process HsLC3A. Interestingly, in Nicotiana benthamiana plants the yeast Atg8 is processed compared to HsLC3A. However, HsLC3A is processed when coexpressed with HsATG4B in plants. Molecular modeling indicates that lack of processing of HsLC3A by plant and yeast ATG4 is not due to lack of interaction with HsLC3A. Our in-depth analyses of ATG4 and ATG8 in the plant lineage combined with results of cross-kingdom ATG8 processing by ATG4 further support the evolutionarily conserved maturation of ATG8. Broad ATG8 processing by HsATG4B and lack of processing of HsLC3A by yeast and plant ATG4s suggest that the cross-kingdom ATG8 processing is determined by ATG8 sequence rather than ATG4.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.