Evidence map›Paper›PMID 27540116›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2016

Clathrin light chains' role in selective endocytosis influences antibody isotype switching.

Shuang Wu, Sophia R Majeed, Timothy M Evans, Marine D Camus, Nicole M L Wong, Yvette Schollmeier, Minjong Park, Jagan R Muppidi, Andrea Reboldi, Peter Parham and 2 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 36 citations in OpenAlex.

  1. Article
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  11. Review
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  13. Clathrin Light Chains: Not to Be Taken so Lightly.Frontiers in cell and developmental biology · 2021
    Review
  14. Clathrin light chain diversity regulates membrane deformation in vitro and synaptic vesicle formation in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  15. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 2 countries.

Shuang WuDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA 94143; Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143; Department of Microbiology and Immunology, University of California, San Francisco, CA 94143; The G. W. Hooper Foundation, University of California, San Francisco, CA 94143;
Sophia R MajeedDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA 94143; Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143; Department of Microbiology and Immunology, University of California, San Francisco, CA 94143; The G. W. Hooper Foundation, University of California, San Francisco, CA 94143;
Timothy M EvansDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA 94143; Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143; Department of Microbiology and Immunology, University of California, San Francisco, CA 94143; The G. W. Hooper Foundation, University of California, San Francisco, CA 94143;
Marine D CamusDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA 94143; Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143; Department of Microbiology and Immunology, University of California, San Francisco, CA 94143; The G. W. Hooper Foundation, University of California, San Francisco, CA 94143; Division of Biosciences, University College London, London WC1E 6BT, United Kingdom;
Nicole M L WongDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA 94143; Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143; Department of Microbiology and Immunology, University of California, San Francisco, CA 94143; The G. W. Hooper Foundation, University of California, San Francisco, CA 94143;
Yvette SchollmeierDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA 94143; Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143; Department of Microbiology and Immunology, University of California, San Francisco, CA 94143; The G. W. Hooper Foundation, University of California, San Francisco, CA 94143;
Minjong ParkDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA 94143; Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143; Department of Microbiology and Immunology, University of California, San Francisco, CA 94143; The G. W. Hooper Foundation, University of California, San Francisco, CA 94143;
Jagan R MuppidiDepartment of Microbiology and Immunology, University of California, San Francisco, CA 94143;
Andrea ReboldiDepartment of Microbiology and Immunology, University of California, San Francisco, CA 94143;
Peter ParhamDepartment of Structural Biology, Stanford University, Stanford, CA 94305; Department of Microbiology and Immunology, Stanford University, Stanford, CA 94305.
Jason G CysterDepartment of Microbiology and Immunology, University of California, San Francisco, CA 94143; f.brodsky@ucl.ac.uk jason.cyster@ucsf.edu.
Frances M BrodskyDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA 94143; Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143; Department of Microbiology and Immunology, University of California, San Francisco, CA 94143; The G. W. Hooper Foundation, University of California, San Francisco, CA 94143; Division of Biosciences, University College London, London WC1E 6BT, United Kingdom; f.brodsky@ucl.ac.uk jason.cyster@ucsf.edu.
University of California, San Francisco · USSan Francisco Foundation · USStanford University · USUniversity College London · GB

Funding

Chemokines and Lymphoid Tissue Organization and FunctionR01AI045073 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI CYSTER, JASON G · 1999 to 2023
$7.6M
PHARMACEUTICAL SCIENCES AND PHARMACOGENOMICST32GM007175 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI AHITUV, NADAV, KROETZ, DEANNA L · 1985 to 2021
$7.1M
STRUCTURAL BIOLOGY OF HLA-A,B ANTIGENSR01AI017892 · NIAID · STANFORD UNIVERSITY · PI PARHAM, PETER R · 1985 to 2019
$6.1M
Research Training in Childhood CancerT32CA128583 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Eric Alejandro Sweet-Cordero · 2007 to 2026
$6.0M
THE MOLECULAR MECHANISM OF CLATHRIN ASSEMBLYR01GM038093 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI BRODSKY, FRANCES M. · 1988 to 2014
$5.0M
The role of clathrin and the Hip proteins in breast cancer metastasisF31CA171594 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MAJEED, SOPHIA · 2012 to 2014
$112k
NCI NIH HHS F31 CA171594NCI NIH HHS T32 CA128583NIAID NIH HHS R01 AI017892NIAID NIH HHS R01 AI045073NIGMS NIH HHS R01 GM038093NIGMS NIH HHS T32 GM007175Wellcome Trust
6 · The paper itself

Abstract

Clathrin, a cytosolic protein composed of heavy and light chain subunits, assembles into a vesicle coat, controlling receptor-mediated endocytosis. To establish clathrin light chain (CLC) function in vivo, we engineered mice lacking CLCa, the major CLC isoform in B lymphocytes, generating animals with CLC-deficient B cells. In CLCa-null mice, the germinal centers have fewer B cells, and they are enriched for IgA-producing cells. This enhanced switch to IgA production in the absence of CLCa was attributable to increased transforming growth factor β receptor 2 (TGFβR2) signaling resulting from defective endocytosis. Internalization of C-X-C chemokine receptor 4 (CXCR4), but not CXCR5, was affected in CLCa-null B cells, and CLC depletion from cell lines affected endocytosis of the δ-opioid receptor, but not the β2-adrenergic receptor, defining a role for CLCs in the uptake of a subset of signaling receptors. This instance of clathrin subunit deletion in vertebrates demonstrates that CLCs contribute to clathrin's role in vivo by influencing cargo selectivity, a function previously assigned exclusively to adaptor molecules.

Indexed as

Gene DeletionImmunoglobulin Class SwitchingAnimalsB-LymphocytesCerebral CortexClathrin Light ChainsEndocytosisGene Expression RegulationHumansImmunoglobulin ALiverMiceMice, Inbred C57BLMice, KnockoutMyocardiumOrgan SpecificityClathrin Light ChainsCXCR4 protein, mouseImmunoglobulin AProtein Serine-Threonine KinasesReceptors, Adrenergic, beta-2Receptors, CXCR4Receptors, Opioid, deltaReceptors, Transforming Growth Factor betaReceptor, Transforming Growth Factor-beta Type IIantibody isotype switchclathrin light chainendocytosisG protein-coupled receptorsTGFβ

Identifiers

PMID27540116
PMCPMC5024586
OpenAlexW2517871575

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.