ArticleScientific reports2016
Creation of an engineered APC system to explore and optimize the presentation of immunodominant peptides of major allergens.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- Whole-protein screening and multi-modal profiling of antigen-specific CD4Nature communications · 2026Article
- High-throughput discovery of MHC class I- and II-restricted T cell epitopes using synthetic cellular circuits.Nature biotechnology · 2025Article
- Assessing the functional potential of conditioned media derived from amniotic epithelial stem cells engineered on 3D biomimetic scaffolds: An in vitro model for tendon regeneration.Materials today. Bio · 2024Article
- T-FINDER: A highly sensitive, pan-HLA platform for functional T cell receptor and ligand discovery.Science advances · 2024Article
- A CMV-induced adaptive human Vδ1+ γδ T cell clone recognizes HLA-DR.The Journal of experimental medicine · 2022Article
- A Highly Sensitive Cell-Based TLR Reporter Platform for the Specific Detection of Bacterial TLR Ligands.Frontiers in immunology · 2021Article
- Abundance and Stability as Common Properties of Allergens.Frontiers in allergy · 2021Review
- Analyzing the Mycobacterium tuberculosis immune response by T-cell receptor clustering with GLIPH2 and genome-wide antigen screening.Nature biotechnology · 2020Article
- Distinct editing functions of natural HLA-DM allotypes impact antigen presentation and CD4Cellular & molecular immunology · 2020Article
- A T-cell reporter platform for high-throughput and reliable investigation of TCR function and biology.Clinical & translational immunology · 2020Article
- Therapeutic PD-L1 antibodies are more effective than PD-1 antibodies in blocking PD-1/PD-L1 signaling.Scientific reports · 2019Article
- Generation of a Jurkat-based fluorescent reporter cell line to evaluate lipid antigen interaction with the human iNKT cell receptor.Scientific reports · 2019Article
- Functional TCR T cell screening using single-cell droplet microfluidics.Lab on a chip · 2018Article
- PD-1 has a unique capacity to inhibit allergen-specific human CD4Scientific reports · 2018Article
- A Jurkat 76 based triple parameter reporter system to evaluate TCR functions and adoptive T cell strategies.Oncotarget · 2018Article
- Not All Immune Checkpoints Are Created Equal.Frontiers in immunology · 2018Review
- Article
- A human monocytic NF-κB fluorescent reporter cell line for detection of microbial contaminants in biological samples.PloS one · 2017Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have generated engineered APC to present immunodominant peptides derived from the major aero-allergens of birch and mugwort pollen, Bet v 1142-153 and Art v 125-36, respectively. Jurkat-based T cell reporter lines expressing the cognate allergen-specific T cell receptors were used to read out the presentation of allergenic peptides on the engineered APC. Different modalities of peptide loading and presentation on MHC class II molecules were compared. Upon exogenous loading with allergenic peptides, the engineered APC elicited a dose-dependent response in the reporter T cells and the presence of chemical loading enhancers strongly increased reporter activation. Invariant chain-based MHC class II targeting strategies of endogenously expressed peptides resulted in stronger activation of the reporters than exogenous loading. Moreover, we used Bet v 1 as model allergen to study the ability of K562 cells to present antigenic peptides derived from whole proteins either taken up or endogenously expressed as LAMP-1 fusion protein. In both cases the ability of these cells to process and present peptides derived from whole proteins critically depended on the expression of HLA-DM. We have identified strategies to achieve efficient presentation of allergenic peptides on engineered APC and demonstrate their use to stimulate T cells from allergic individuals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.