ArticleScientific reports2016
Solid-state NMR characterization of amphomycin effects on peptidoglycan and wall teichoic acid biosyntheses in Staphylococcus aureus.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Pardon the interruption: how Und-P sequestration has reshaped our understanding of the bacterial cell envelope.Journal of bacteriology · 2026Review
- Novel Synthetic Strategies Towards Analogues of Cadaside and Malacidin Antibiotic Peptides.Biomolecules · 2025Article
- An Overview of Microorganisms Immobilized in a Gel Structure for the Production of Precursors, Antibiotics, and Valuable Products.Gels (Basel, Switzerland) · 2024Review
- The effects of daptomycin on cell wall biosynthesis in Enterococcal faecalis.Scientific reports · 2023Article
- Detection of intact vancomycin-arginine as the active antibacterial conjugate inRSC medicinal chemistry · 2023Article
- Article
- Bioinformatic prospecting and synthesis of a bifunctional lipopeptide antibiotic that evades resistance.Science (New York, N.Y.) · 2022Article
- Solid-State NMR Investigations of Extracellular Matrixes and Cell Walls of Algae, Bacteria, Fungi, and Plants.Chemical reviews · 2022Review
- Tackling Antibiotic Resistance with Compounds of Natural Origin: A Comprehensive Review.Biomedicines · 2020Review
- Chemical Biology Tools for Examining the Bacterial Cell Wall.Cell chemical biology · 2020Review
- The calcium-dependent lipopeptide antibiotics: structure, mechanism, & medicinal chemistry.MedChemComm · 2019Review
- Crystallomycin revisited after 60 years: aspartocins B and C.MedChemComm · 2018Article
- Peptidoglycan Compositional Analysis of Enterococcus faecalis Biofilm by Stable Isotope Labeling by Amino Acids in a Bacterial Culture.Biochemistry · 2018Article
- Inhibition of d-Ala incorporation into wall teichoic acid in Staphylococcus aureus by desleucyl-oritavancin.Chemical communications (Cambridge, England) · 2017Article
- Hidden Mode of Action of Glycopeptide Antibiotics: Inhibition of Wall Teichoic Acid Biosynthesis.The journal of physical chemistry. B · 2017Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Amphomycin and MX-2401 are cyclic lipopeptides exhibiting bactericidal activities against Gram-positive pathogens. Amphomycin and MX-2401 share structural similarities with daptomycin, but unlike daptomycin they do not target bacterial membrane. In this study, we investigate in vivo modes of action for amphomycin and MX-2401 in intact whole cells of Staphylococcus aureus by measuring the changes of peptidoglycan and wall teichoic acid compositions using solid-state NMR. S. aureus were grown in a defined media containing isotope labels [1-(13)C]glycine and L-[ε-(15)N]lysin, L-[1-(13)C]lysine and D-[(15)N]alanine, or D-[1-(13)C]alanine and [(15)N]glycine, to selectively (13)C-(15)N pair label peptidoglycan bridge-link, stem-link, and cross-link, respectively. (13)C{(15)N} and (15)N{(13)C} rotational-echo double resonance NMR measurements determined that cyclic lipopeptide-treated S. aureus exhibited thinning of the cell wall, accumulation of Park's nucleotide, inhibition of glycine utilization for purine biosynthesis, reduction of ester-linked D-Ala in teichoic acids, and reduction of peptidoglycan cross-linking. Whole cell NMR analysis also revealed that S. aureus, in presence of amphomycin and MX-2401, maintained the incorporation of D-Ala during peptidoglycan biosynthesis while the incorporation of D-Ala into teichoic acids was inhibited. These effects are consistent with amphomycin's dual inhibition of both peptidoglycan and wall teichoic acid biosyntheses in S. aureus.
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