ArticleTissue engineering. Part C, Methods2016
A Flow Perfusion Bioreactor System for Vocal Fold Tissue Engineering Applications.
Article in Tissue engineering. Part C, Methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Flexible 3D-Printable Materials for Application in Medical Research: A Comprehensive Comparison of Commercially Available Materials.3D printing and additive manufacturing · 2025Article
- A hybrid vocal fold phonatory platform for pediatric phonation modeling.Frontiers in bioengineering and biotechnology · 2025Article
- Biophysical aspects of mechanotransduction in cells and their physiological/biological implications in vocal fold vibration: a narrative review.Frontiers in cell and developmental biology · 2025Review
- Synthetic, self-oscillating vocal fold models for voice production researcha).The Journal of the Acoustical Society of America · 2024Review
- Article
- Flow-induced oscillations of vocal-fold replicas with tuned extensibility and material properties.Scientific reports · 2023Article
- Functional Analysis of Injectable Substance Treatment on Surgically Injured Rabbit Vocal Folds.Journal of voice : official journal of the Voice Foundation · 2023Article
- Optimization of Synthetic Vocal Fold Models for Glottal Closure.Journal of engineering and science in medical diagnostics and therapy · 2022Article
- Bioreactors for Vocal Fold Tissue Engineering.Tissue engineering. Part B, Reviews · 2022Review
- Injectable, Pore-Forming, Perfusable Double-Network Hydrogels Resilient to Extreme Biomechanical Stimulations.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- In vitro mechanical vibration down-regulates pro-inflammatory and pro-fibrotic signaling in human vocal fold fibroblasts.PloS one · 2020Article
- Magnetic resonance imaging-based measurement of internal deformation of vibrating vocal fold models.The Journal of the Acoustical Society of America · 2019Article
- Mechanotransduction of vocal fold fibroblasts and mesenchymal stromal cells in the context of the vocal fold mechanome.Journal of biomechanics · 2019Article
- Article
- A tissue-mimetic nano-fibrillar hybrid injectable hydrogel for potential soft tissue engineering applications.Scientific reports · 2018Article
- High-Performance Agent-Based Modeling Applied to Vocal Fold Inflammation and Repair.Frontiers in physiology · 2018Article
- Electrically conductive synthetic vocal fold replicas for voice production research.The Journal of the Acoustical Society of America · 2017Article
- In vitro fibrillogenesis of tropocollagen type III in collagen type I affects its relative fibrillar topology and mechanics.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
The human vocal folds (VFs) undergo complex biomechanical stimulation during phonation. The aim of the present study was to develop and validate a phono-mimetic VF flow perfusion bioreactor, which mimics the mechanical microenvironment of the human VFs in vitro. The bioreactor uses airflow-induced self-oscillations, which have been shown to produce mechanical loading and contact forces that are representative of human phonation. The bioreactor consisted of two synthetic VF replicas within a silicone body. A cell-scaffold mixture (CSM) consisting of human VF fibroblasts, hyaluronic acid, gelatin, and a polyethylene glycol cross-linker was injected into cavities within the replicas. Cell culture medium (CCM) was perfused through the scaffold by using a customized secondary flow loop. After the injection, the bioreactor was operated with no stimulation over a 3-day period to allow for cell adaptation. Phonation was subsequently induced by using a variable speed centrifugal blower for 2 h each day over a period of 4 days. A similar bioreactor without biomechanical stimulation was used as the nonphonatory control. The CSM was harvested from both VF replicas 7 days after the injection. The results confirmed that the phono-mimetic bioreactor supports cell viability and extracellular matrix proteins synthesis, as expected. Many scaffold materials were found to degrade because of challenges from phonation-induced biomechanical stimulation as well as due to biochemical reactions with the CCM. The bioreactor concept enables future investigations of the effects of different phonatory characteristics, that is, voice regimes, on the behavior of the human VF cells. It will also help study the long-term functional outcomes of the VF-specific biomaterials before animal and clinical studies.
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