ArticlePloS one2016
The O-Antigen Flippase Wzk Can Substitute for MurJ in Peptidoglycan Synthesis in Helicobacter pylori and Escherichia coli.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Analysis of essential genes inJournal of bacteriology · 2025Article
- Analysis of Essential Genes inbioRxiv : the preprint server for biology · 2025Article
- Massively parallel barcode sequencing revealed the interchangeability of capsule transporters inScience advances · 2025Article
- Rewiring the pneumococcal capsule pathway for investigating glycosyltransferase specificity and genetic glycoengineering.Science advances · 2023Article
- Bacteroid Development, Transcriptome, and Symbiotic Nitrogen-Fixing Comparison of Bradyrhizobium arachidis in Nodules of Peanut (Arachis hypogaea) and Medicinal Legume Sophora flavescens.Microbiology spectrum · 2023Article
- Structure and Mechanism of the Lipid Flippase MurJ.Annual review of biochemistry · 2022Review
- The Bacterial Cell Wall: From Lipid II Flipping to Polymerization.Chemical reviews · 2022Review
- Use of Mutagenesis and Functional Screens to Characterize Essential Genes Involved in Lipopolysaccharide Transport.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Article
- A genetic screen to identify factors affected by undecaprenyl phosphate recycling uncovers novel connections to morphogenesis in Escherichia coli.Molecular microbiology · 2021Article
- Regulation of peptidoglycan synthesis and remodelling.Nature reviews. Microbiology · 2020Review
- The bacterial lipid II flippase MurJ functions by an alternating-access mechanism.The Journal of biological chemistry · 2019Article
- Structure and mutagenic analysis of the lipid II flippase MurJ fromProceedings of the National Academy of Sciences of the United States of America · 2018Article
- The transpeptidase PbpA and noncanonical transglycosylase RodA ofThe Journal of biological chemistry · 2018Article
- Don't let sleeping dogmas lie: new views of peptidoglycan synthesis and its regulation.Molecular microbiology · 2017Review
- A viral protein antibiotic inhibits lipid II flippase activity.Nature microbiology · 2017Article
- Interplay between Penicillin-binding proteins and SEDS proteins promotes bacterial cell wall synthesis.Scientific reports · 2017Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
The peptidoglycan (PG) cell wall is an essential component of the cell envelope of most bacteria. Biogenesis of PG involves a lipid-linked disaccharide-pentapeptide intermediate called lipid II, which must be translocated across the cytoplasmic membrane after it is synthesized in the inner leaflet of this bilayer. Accordingly, it has been demonstrated that MurJ, the proposed lipid II flippase in Escherichia coli, is required for PG biogenesis, and thereby viability. In contrast, MurJ is not essential in Bacillus subtilis because this bacterium produces AmJ, an unrelated protein that is functionally redundant with MurJ. In this study, we investigated why MurJ is not essential in the prominent gastric pathogen, Helicobacter pylori. We found that in this bacterium, Wzk, the ABC (ATP-binding cassette) transporter that flips the lipid-linked O- or Lewis- antigen precursors across the inner membrane, is redundant with MurJ for cell viability. Heterologous expression of wzk in E. coli also suppresses the lethality caused by the loss of murJ. Furthermore, we show that this cross-species complementation is abolished when Wzk is inactivated by mutations that target a domain predicted to be required for ATPase activity. Our results suggest that Wzk can flip lipid II, implying that Wzk is the flippase with the most relaxed specificity for lipid-linked saccharides ever identified.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.