Comparative In silico Study of Sex-Determining Region Y (SRY) Protein Sequences Involved in Sex-Determining.

Masoume Vakili Azghandi, Mohammadreza Nasiri, Ali Shamsa, Mohsen Jalali, Mohammad Mahdi Shariati

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Article in Reports of biochemistry & molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1 citing paper in PubMed.

Noninvasive Fetal Sex Determination by Real-Time PCR and TaqMan Probes.Reports of biochemistry & molecular biology · 2020
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Masoume Vakili AzghandiDepartment of Animal Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.

Mohammadreza NasiriDepartment of Animal Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.

Ali ShamsaDepartment of Urology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Mohsen JalaliDepartment of Urology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Mohammad Mahdi ShariatiDepartment of Animal Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.

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6 · The paper itself

Abstract

backgroundThe SRY gene (SRY) provides instructions for making a transcription factor called the sex-determining region Y protein. The sex-determining region Y protein causes a fetus to develop as a male. In this study, SRY of 15 spices included of human, chimpanzee, dog, pig, rat, cattle, buffalo, goat, sheep, horse, zebra, frog, urial, dolphin and killer whale were used for determine of bioinformatic differences.

methodsNucleotide sequences of SRY were retrieved from the NCBI databank. Bioinformatic analysis of SRY is done by CLC Main Workbench version 5.5 and ClustalW (http:/www.ebi.ac.uk/clustalw/) and MEGA6 softwares.

resultsThe multiple sequence alignment results indicated that SRY protein sequences from Orcinus orca (killer whale) and Tursiopsaduncus (dolphin) have least genetic distance of 0.33 in these 15 species and are 99.67% identical at the amino acid level. Homosapiens and Pantroglodytes (chimpanzee) have the next lowest genetic distance of 1.35 and are 98.65% identical at the amino acid level.

conclusionThese findings indicate that the SRY proteins are conserved in the 15 species, and their evolutionary relationships are similar.

Indexed as

Bioinformatics analysisPhylogenySRY gene

Identifiers

PMID27536700

PMCPMC4986265

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