ArticleFrontiers in microbiology2016
An Alkaline Protease from Bacillus pumilus MP 27: Functional Analysis of Its Binding Model toward Its Applications As Detergent Additive.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 102 citations in OpenAlex.
- Enzymatic recovery of nano-silver from used X-ray films by alkaline protease fromBioengineered · 2025Article
- Purification and characterization of an extremely thermostable metalloprotease from Geobacillus thermoleovorans HBB208.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Stable protease from Bacillus licheniformis-MA1 strain: statistical production optimization, kinetic and thermodynamic characterization, and application in silver recovery from used X-ray films.Microbial cell factories · 2025Article
- Comprehensive biochemical, molecular and structural characterization of subtilisin with fibrinolytic potential in bioprocessing.Bioresources and bioprocessing · 2025Article
- Exploring newly isolated thermotolerant, halotolerant and antimicrobial resistant Bacillus subtilis ProNTL1 from tannery waste and its alkaline protease production.Molecular biology reports · 2024Article
- New Bacillus paralicheniformis strain with high proteolytic and keratinolytic activity.Scientific reports · 2024Article
- Computational analysis to comprehend the structure-function properties of fibrinolytic enzymes fromHeliyon · 2024Article
- Alkaline Thermo- and Oxidant-Stable Protease fromIndian journal of microbiology · 2023Article
- Increased Expression Levels of Thermophilic Serine Protease TTHA0724 through Signal Peptide Screening inInternational journal of molecular sciences · 2023Article
- Isolation and Characterization ofInternational journal of molecular sciences · 2023Article
- Bacillus Swezeyi B2 Strain: A Novel Alkaliphilic Bacterium Producer of Alkaline-, Thermal, Oxidant-, and Surfactant-Stable Protease, Extremely Efficient in Detergency.Current microbiology · 2023Article
- Isolation and identification of protease-producingFrontiers in bioengineering and biotechnology · 2023Article
- Response of selected microbial strains and their consortia to the presence of automobile paints: Biofilm growth, matrix protein content and hydrolytic enzyme activity.Saudi journal of biological sciences · 2022Article
- Overexpression ofFrontiers in microbiology · 2022Article
- Microbial proteases: ubiquitous enzymes with innumerable uses.3 Biotech · 2021Review
- Article
- Isolation and characterization of a new cold-active protease from psychrotrophic bacteria of Western Himalayan glacial soil.Scientific reports · 2021Article
- Biochemical Characterization and Functional Analysis of Heat Stable High Potential Protease ofBiomolecules · 2021Article
- Characterization of a Protease Hyper-Productive Mutant of Bacillus pumilus by Comparative Genomic and Transcriptomic Analysis.Current microbiology · 2020Article
- Proteolytic bacteria isolated from agro-waste dumpsites produced keratinolytic enzymes.Biotechnology reports (Amsterdam, Netherlands) · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A proteolytic strain of Bacillus pumilus MP 27 was isolated from water samples of Southern ocean produced alkaline protease. Since protease production need expensive ingredients, an economically viable process was developed by using low cost carbon source, wheat straw, supplemented with peptone. This protease was active within temperature ranges 10-70°C at pH 9. This process was optimized by response surface methodology using a Box Bekhman design by Design Expert 7.0 software that increased the protease activity to 776.5 U/ml. Moreover, the enzyme was extremely stable at a broad range of temperature and pH retaining 69% of its activity at 50°C and 70% at pH 11. The enzyme exhibited excellent compatibility with surfactants and commercial detergents, showing 87% stability with triton X-100 and 100% stability with Tide commercial detergent. The results of the wash performance analysis demonstrated considerably good de-staining at 50 and 4°C with low supplementation (109 U/ml). Molecular modeling of the protease revealed the presence of serine proteases, subtilase family and serine active site and further docking supported the association of catalytic site with the various substrates. Certainly, such protease can be considered as a good detergent additive in detergent industry with a possibility to remove the stains effectively even in a cold wash.
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Registered trials
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