ArticleFrontiers in microbiology2016
Quantification of Hepatitis E Virus in Naturally-Contaminated Pig Liver Products.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Real-Time PCR-Based Detection of Hepatitis E Virus in Groundwater: Primer Performance and Method Validation.International journal of molecular sciences · 2025Article
- Development and validation of nanoplate-based RT-dPCR assay for canine respiratory coronavirus detection in various clinical samples.BMC veterinary research · 2025Article
- Hepatitis E and Potential Public Health Implications from a One-Health Perspective: Special Focus on the European Wild Boar (Pathogens (Basel, Switzerland) · 2024Review
- Article
- Development of a Sensitive and Specific Quantitative RT-qPCR Method for the Detection of Hepatitis E Virus Genotype 3 in Porcine Liver and Foodstuff.Foods (Basel, Switzerland) · 2024Article
- A Multifaceted Approach for Evaluating Hepatitis E Virus Infectivity In Vitro: Cell Culture and Innovative Molecular Methods for Integrity Assessment.Veterinary sciences · 2023Article
- Review
- Prevalence of Foodborne Viruses in Berries Harvested in Canada.Foods (Basel, Switzerland) · 2023Article
- Inactivation of foodborne viruses by novel organic peroxyacid-based disinfectants.Frontiers in microbiology · 2023Article
- Real-Time PCR-Based Methods for Detection of Hepatitis E Virus in Pork Products: A Critical Review.Microorganisms · 2022Review
- Development of a Tetraplex qPCR for the Molecular Identification and Quantification of Human Enteric Viruses, NoV and HAV, in Fish Samples.Microorganisms · 2021Article
- Application of Lab-on-Chip for Detection of Microbial Nucleic Acid in Food and Environment.Frontiers in microbiology · 2021Review
- Assessment of the Presence of Hepatitis E virus in Surface Water and Drinking Water in Portugal.Microorganisms · 2020Article
- Increased Mast Cell Activation in Mongolian Gerbils Infected by Hepatitis E Virus.Frontiers in microbiology · 2018Article
- Viability RT-qPCR to Distinguish Between HEV and HAV With Intact and Altered Capsids.Frontiers in microbiology · 2018Article
- Detection of Astrovirus, Rotavirus C, and Hepatitis E Viral RNA in Adult and Juvenile Farmed Mink (Frontiers in veterinary science · 2018Article
- Recent knowledge on hepatitis E virus in Suidae reservoirs and transmission routes to human.Veterinary research · 2017Review
- Public health risks associated with hepatitis E virus (HEV) as a food-borne pathogen.EFSA journal. European Food Safety Authority · 2017Article
- Prevalence and Molecular Characterization of the Hepatitis E Virus in Retail Pork Products Marketed in Canada.Food and environmental virology · 2017Article
- Nanofluidic digital PCR for the quantification of Norovirus for water quality assessment.PloS one · 2017Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hepatitis E virus (HEV), the cause of self-limiting acute hepatitis in humans, is widespread and endemic in many parts of the world. The foodborne transmission of HEV has become of concern due to the identification of undercooked pork products as a risk factor for infection. Foodborne enteric viruses are conventionally processed by quantitative RT-PCR (RT-qPCR), which gives sensitive and quantitative detection results. Recently, digital PCR (dPCR) has been described as a novel approach to genome quantification with no need for a standard curve. The performance of microfluidic digital RT-PCR (RT-dPCR) was compared to RT-qPCR when detecting HEV in pig liver products. The sensitivity of the RT-dPCR assay was similar to that of RT-qPCR, and quantitative data obtained by both detection methods were not significantly different for almost all samples. This absolute quantification approach may be useful for standardizing quantification of HEV in food samples and may be extended to quantifying other human pathogens in food samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.