ArticleFrontiers in cellular neuroscience2016
Epigenetic DNA Demethylation Causes Inner Ear Stem Cell Differentiation into Hair Cell-Like Cells.
Article in Frontiers in cellular neuroscience, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- The role of epigenetic modifications in sensory hair cell development, survival, and regulation.Frontiers in cellular neuroscience · 2023Review
- Dnmt1 is required for the development of auditory organs via cell cycle arrest and Fgf signalling.Cell proliferation · 2022Article
- Inhibiting DNA methylation alleviates cisplatin-induced hearing loss by decreasing oxidative stress-induced mitochondria-dependent apoptosisActa pharmaceutica Sinica. B · 2022Article
- Hearing Recovery Induced by DNA Demethylation in a Chemically Deafened Adult Mouse Model.Frontiers in cellular neuroscience · 2022Article
- Molecular Pathways Modulating Sensory Hair Cell Regeneration in Adult Mammalian Cochleae: Progress and Perspectives.International journal of molecular sciences · 2021Review
- Transcriptional epigenetic regulation of Fkbp1/Pax9 genes is associated with impaired sensitivity to platinum treatment in ovarian cancer.Clinical epigenetics · 2021Article
- Review
- Generation of Cochlear Hair Cells from Sox2 Positive Supporting Cells via DNA Demethylation.International journal of molecular sciences · 2020Article
- Identification of Neural Stem Cells from Postnatal Mouse Auditory Cortex In Vitro.Stem cells and development · 2019Article
- Generation of new hair cells by DNA methyltransferase (Dnmt) inhibitor 5-azacytidine in a chemically-deafened mouse model.Scientific reports · 2019Article
- Approaches for the study of epigenetic modifications in the inner ear and related tissues.Hearing research · 2019Review
- Insights into inner ear-specific gene regulation: Epigenetics and non-coding RNAs in inner ear development and regeneration.Seminars in cell & developmental biology · 2017Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The DNA methyltransferase (DNMT) inhibitor 5-azacytidine (5-aza) causes genomic demethylation to regulate gene expression. However, it remains unclear whether 5-aza affects gene expression and cell fate determination of stem cells. In this study, 5-aza was applied to mouse utricle sensory epithelia-derived progenitor cells (MUCs) to investigate whether 5-aza stimulated MUCs to become sensory hair cells. After treatment, MUCs increased expression of hair cell genes and proteins. The DNA methylation level (indicated by percentage of 5-methylcytosine) showed a 28.57% decrease after treatment, which causes significantly repressed DNMT1 protein expression and DNMT activity. Additionally, FM1-43 permeation assays indicated that the permeability of 5-aza-treated MUCs was similar to that of sensory hair cells, which may result from mechanotransduction channels. This study not only demonstrates a possible epigenetic approach to induce tissue specific stem/progenitor cells to become sensory hair cell-like cells, but also provides a cell model to epigenetically modulate stem cell fate determination.
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