ArticleProceedings of the National Academy of Sciences of the United States of America2016
Superresolution intrinsic fluorescence imaging of chromatin utilizing native, unmodified nucleic acids for contrast.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Solvatochromic BODIPYs with Polarity-Independent Absorption for Functional Super-resolution Imaging of Live Cell Membranes.Chemical & biomedical imaging · 2026Article
- Spectroscopic Super-resolution Imaging of DNA Molecules using Intrinsic Contrast.Journal of visualized experiments : JoVE · 2026Article
- Ultra-weak photon emission: from oxidative metabolism to DNA-based communication: a review of biochemical, biophysical and quantum biological perspectives.Frontiers in endocrinology · 2026Review
- Aggregation-induced emission of DNA fluorescence as a novel pan-marker of cell death, senescence and sepsisTheranostics · 2026Article
- Label-free structural imaging of plant roots and microbes using third-harmonic generation microscopy.Scientific reports · 2025Article
- Chip-based label-free incoherent super-resolution optical microscopy.Light, science & applications · 2025Article
- Review
- Ultra-weak photon emission from DNA.Scientific reports · 2024Article
- Deep learning-based spectroscopic single-molecule localization microscopy.Journal of biomedical optics · 2024Article
- Molecular histopathology of matrix proteins through autofluorescence super-resolution microscopy.Scientific reports · 2024Article
- Dissection of DNA damage and repair pathways in live cells by femtosecond laser microirradiation and free-electron modeling.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Viewing life without labels under optical microscopes.Communications biology · 2023Review
- Spectroscopic single-molecule localization microscopy: applications and prospective.Nano convergence · 2023Review
- Review
- Article
- Article
- Contribution of advanced fluorescence nano microscopy towards revealing mitotic chromosome structure.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Review
- Cryo-nanoscale chromosome imaging-future prospects.Biophysical reviews · 2020Review
- Three-Dimensional Single-Molecule Localization Microscopy in Whole-Cell and Tissue Specimens.Annual review of biomedical engineering · 2020Review
- Tracking and Predicting Human Somatic Cell Reprogramming Using Nuclear Characteristics.Biophysical journal · 2020Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Visualizing the nanoscale intracellular structures formed by nucleic acids, such as chromatin, in nonperturbed, structurally and dynamically complex cellular systems, will help expand our understanding of biological processes and open the next frontier for biological discovery. Traditional superresolution techniques to visualize subdiffractional macromolecular structures formed by nucleic acids require exogenous labels that may perturb cell function and change the very molecular processes they intend to study, especially at the extremely high label densities required for superresolution. However, despite tremendous interest and demonstrated need, label-free optical superresolution imaging of nucleotide topology under native nonperturbing conditions has never been possible. Here we investigate a photoswitching process of native nucleotides and present the demonstration of subdiffraction-resolution imaging of cellular structures using intrinsic contrast from unmodified DNA based on the principle of single-molecule photon localization microscopy (PLM). Using DNA-PLM, we achieved nanoscopic imaging of interphase nuclei and mitotic chromosomes, allowing a quantitative analysis of the DNA occupancy level and a subdiffractional analysis of the chromosomal organization. This study may pave a new way for label-free superresolution nanoscopic imaging of macromolecular structures with nucleotide topologies and could contribute to the development of new DNA-based contrast agents for superresolution imaging.
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