ArticleJournal of clinical microbiology2016
Multicenter Evaluation of BD Max Enteric Parasite Real-Time PCR Assay for Detection of Giardia duodenalis, Cryptosporidium hominis, Cryptosporidium parvum, and Entamoeba histolytica.
Article in Journal of clinical microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 61 citations in OpenAlex.
- Current and emerging molecular diagnostic approaches in the detection of human parasites.Parasitology research · 2026Review
- Multiplex Polymerase Chain Reaction Panels for Gastrointestinal Infections: Current Evidence, Regulatory Hurdles, and the Way Forward.Open forum infectious diseases · 2025Article
- Performance validation of the BD MAX Enteric Parasite Panel using simulated samples in low endemic regions.Parasites, hosts and diseases · 2025Article
- Evaluation of the Use of Singleplex and Duplex CerTest VIASURE Real-Time PCR Assays to Detect Common Intestinal Protist Parasites.Diagnostics (Basel, Switzerland) · 2024Article
- Development, Optimisation and Validation of a Novel Multiplex Real-Time PCR Method for the Simultaneous Detection ofPathogens (Basel, Switzerland) · 2022Article
- Evaluation of a Novel Commercial Real-Time PCR Assay for the Simultaneous Detection ofMicrobiology spectrum · 2022Article
- Selecting a multiplex PCR panel for accurate molecular diagnosis of intestinal protists: a comparative study of AllplexParasite (Paris, France) · 2022Article
- Parasitic infections among patients hospitalized in the Tropical and Parasitic Clinic of Poznan University of Medical Sciences, Poland between 2015 and 2018. Is there a relationship between protozoa infection and gastrointestinal symptoms?Przeglad gastroenterologiczny · 2022Article
- Comparison of Three Real-Time PCR Assays Targeting the SSU rRNA Gene, the COWP Gene and the DnaJ-Like Protein Gene for the Diagnosis ofPathogens (Basel, Switzerland) · 2021Article
- Review
- Comparative Performance of Eight PCR Methods to DetectPathogens (Basel, Switzerland) · 2021Article
- Article
- DetectingThe American journal of tropical medicine and hygiene · 2020Article
- Evaluation of the AllplexMicroorganisms · 2020Article
- Performance of a new molecular assay for the detection of gastrointestinal pathogens.Access microbiology · 2020Article
- Multicenter Clinical Validation of the Molecular BD Max Enteric Viral Panel for Detection of Enteric Pathogens.Journal of clinical microbiology · 2019Article
- Prevalence ofTropical medicine and infectious disease · 2019Article
- Comparison of multiplexed-tandem real-time PCR panel with reference real-time PCR molecular diagnostic assays for detection of Giardia intestinalis and Tritrichomonas foetus in cats.Veterinary parasitology · 2019Article
- Article
- Use and Interpretation of Enteropathogen Multiplex Nucleic Acid Amplification Tests in Patients With Suspected Infectious Diarrhea.Gastroenterology & hepatology · 2018Article
Corrections and comments
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Authors and funding
11 authors at 9 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Common causes of chronic diarrhea among travelers worldwide include protozoan parasites. The majority of parasitic infections are caused by Giardia duodenalis, Entamoeba histolytica, Cryptosporidium parvum, and Cryptosporidium hominis Similarly, these species cause the majority of parasitic diarrhea acquired in the United States. Detection of parasites by gold standard microscopic methods is time-consuming and requires considerable expertise; enzyme immunoassays and direct fluorescent-antibody (DFA) stains have lowered hands-on time for testing, but improvements in sensitivity and technical time may be possible with a PCR assay. We performed a clinical evaluation of a multiplex PCR panel, the enteric parasite panel (EPP), for the detection of these common parasites using the BD Max instrument, which performs automated extraction and amplification. A total of 2,495 compliant specimens were enrolled, including 2,104 (84%) specimens collected prospectively and 391 (16%) specimens collected retrospectively. Approximately equal numbers were received in 10% formalin (1,273 specimens) and unpreserved (1,222 specimens). The results from the EPP were compared to those from alternate PCR and bidirectional sequencing (APCR), as well as DFA (G. duodenalis and C. parvum or C. hominis) or trichrome stain (E. histolytica). The sensitivity and specificity for prospective and retrospective specimens combined were 98.2% and 99.5% for G. duodenalis, 95.5% and 99.6 for C. parvum or C. hominis, and 100% and 100% for E. histolytica, respectively. The performance of the FDA-approved BD Max EPP compared well to the reference methods and may be an appropriate substitute for microscopic examination or immunoassays.
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