Evidence map›Paper›PMID 27535686›Full record

ArticleJournal of clinical microbiology2016

Detection of Anti-Hepatitis B Virus Drug Resistance Mutations Based on Multicolor Melting Curve Analysis.

Yi Mou, Muhammad Ammar Athar, Yuzhen Wu, Ye Xu, Jianhua Wu, Zhenxing Xu, Zulfiqar Hayder, Saeed Khan, Muhammad Idrees, Muhammad Israr Nasir and 2 more

Open access · bronzeAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Hepatitis B Virus: From Diagnosis to Treatment.Polish journal of microbiology · 2020
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 2 countries.

Yi MouState Key Laboratory of Cellular Stress Biology, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Engineering Research Center of Molecular Diagnostics of the Ministry of Education, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
Muhammad Ammar AtharState Key Laboratory of Cellular Stress Biology, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Engineering Research Center of Molecular Diagnostics of the Ministry of Education, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
Yuzhen WuState Key Laboratory of Cellular Stress Biology, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Engineering Research Center of Molecular Diagnostics of the Ministry of Education, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
Ye XuState Key Laboratory of Cellular Stress Biology, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Engineering Research Center of Molecular Diagnostics of the Ministry of Education, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
Jianhua WuXiamen Hospital of Traditional Chinese Medicine, Xiamen, Fujian, China.
Zhenxing XuXiamen Hospital of Traditional Chinese Medicine, Xiamen, Fujian, China.
Zulfiqar HayderDepartment of Pathology, Quid-e-Azam Medical College, Bahawalpur, Punjab, Pakistan.
Saeed KhanDepartment of Molecular Pathology, Dow University of Health Sciences, Karachi, Pakistan.
Muhammad IdreesCenter for Applied Molecular Biology, University of the Punjab, Lahore, Pakistan.
Muhammad Israr NasirDepartment of Molecular Pathology, Liaquat National Hospital, Karachi, Pakistan.
Yiqun LiaoState Key Laboratory of Cellular Stress Biology, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Engineering Research Center of Molecular Diagnostics of the Ministry of Education, School of Life Sciences, Xiamen University, Xiamen, Fujian, China yqliao@xmu.edu.cn qgli@xmu.edu.cn.
Qingge LiState Key Laboratory of Cellular Stress Biology, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Engineering Research Center of Molecular Diagnostics of the Ministry of Education, School of Life Sciences, Xiamen University, Xiamen, Fujian, China yqliao@xmu.edu.cn qgli@xmu.edu.cn.
Xiamen University · CNBahawal Victoria Hospital · PKDow University of Health Sciences · PKLiaquat National Hospital · PKUniversity of the Punjab · PK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Detection of anti-hepatitis B virus (HBV) drug resistance mutations is critical for therapeutic decisions for chronic hepatitis B virus infection. We describe a real-time PCR-based assay using multicolor melting curve analysis (MMCA) that could accurately detect 24 HBV nucleotide mutations at 10 amino acid positions in the reverse transcriptase region of the HBV polymerase gene. The two-reaction assay had a limit of detection of 5 copies per reaction and could detect a minor mutant population (5% of the total population) with the reverse transcriptase M204V amino acid mutation in the presence of the major wild-type population when the overall concentration was 10

Indexed as

Drug Resistance, ViralMutationTransition TemperatureAntiviral AgentsColorCosts and Cost AnalysisDNA, ViralHepatitis B virusHumansReal-Time Polymerase Chain ReactionRNA-Directed DNA PolymeraseStaining and LabelingTime FactorsAntiviral AgentsDNA, ViralRNA-Directed DNA Polymerase

Identifiers

PMID27535686
PMCPMC5078540
OpenAlexW2539180312

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.