ArticleScientific reports2016
Specific polyunsaturated fatty acids modulate lipid delivery and oocyte development in C. elegans revealed by molecular-selective label-free imaging.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 38 citations in OpenAlex.
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- An inducible and reversible system to regulate unsaturated fatty acid biosynthesis in C. elegans.G3 (Bethesda, Md.) · 2025Article
- Broadband coherent anti-Stokes Raman scattering (BCARS) microscopy for rapid, label-free biological imaging.The Review of scientific instruments · 2025Article
- High-glucose diets differentially modulate phosphatidylcholine metabolism and fecundity inFrontiers in cell and developmental biology · 2025Article
- Identifying lipid particle sub-types in liveFrontiers in chemistry · 2023Article
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- Worms, Fat, and Death:Metabolites · 2021Review
- The Mechanism of Traditional Chinese Medicine Based on Semi-Targeted Metabolomics to Improve IVF Outcomes in Senile Patients.Evidence-based complementary and alternative medicine : eCAM · 2021Article
- Reproductive Suicide: Similar Mechanisms of Aging inFrontiers in cell and developmental biology · 2021Article
- Identification of a Novel Link between the Intermediate Filament Organizer IFO-1 and Cholesterol Metabolism in theInternational journal of molecular sciences · 2020Article
- Perfluorooctanesulfonic acid (PFOS) and perfluorobutanesulfonic acid (PFBS) impaired reproduction and altered offspring physiological functions in Caenorhabditis elegans.Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · 2020Article
- Spectroscopic coherent Raman imaging of Caenorhabditis elegans reveals lipid particle diversity.Nature chemical biology · 2020Article
- Antisense Oligonucleotides Used to Identify Telomeric G-Quadruplexes in Metaphase Chromosomes and Fixed Cells by Fluorescence Lifetime Imaging Microscopy ofMolecules (Basel, Switzerland) · 2020Article
- Membrane Fluidity Is Regulated Cell Nonautonomously byGenetics · 2018Article
- Transcriptional activation of zebrafish fads2 promoter and its transient transgene expression in yolk syncytial layer of zebrafish embryos.Scientific reports · 2018Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polyunsaturated fatty acids (PUFAs) exhibit critical functions in biological systems and their importance during animal oocyte maturation has been increasingly recognized. However, the detailed mechanism of lipid transportation for oocyte development remains largely unknown. In this study, the transportation of yolk lipoprotein (lipid carrier) and the rate of lipid delivery into oocytes in live C. elegans were examined for the first time by using coherent anti-Stokes Raman scattering (CARS) microscopy. The accumulation of secreted yolk lipoprotein in the pseudocoelom of live C. elegans can be detected by CARS microscopy at both protein (~1665 cm(-1)) and lipid (~2845 cm(-1)) Raman bands. In addition, an image analysis protocol was established to quantitatively measure the levels of secreted yolk lipoprotein aberrantly accumulated in PUFA-deficient fat mutants (fat-1, fat-2, fat-3, fat-4) and PUFA-supplemented fat-2 worms (the PUFA add-back experiments). Our results revealed that the omega-6 PUFAs, not omega-3 PUFAs, play a critical role in modulating lipid/yolk level in the oocytes and regulating reproductive efficiency of C. elegans. This work demonstrates the value of using CARS microscopy as a molecular-selective label-free imaging technique for the study of PUFA regulation and oocyte development in C. elegans.
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