ArticleMolecular biology of the cell2016
Automated analysis of filopodial length and spatially resolved protein concentration via adaptive shape tracking.
Article in Molecular biology of the cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Semi-automatic geometrical reconstruction and analysis of filopodia dynamics in 4D two-photon microscopy images.BMC bioinformatics · 2026Article
- From cells to pixels: A decision tree for designing bioimage analysis pipelines.Journal of microscopy · 2025Article
- Dendrite tapering actuates a self-organizing signaling circuit for stochastic filopodia initiation in neurons.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- NELL2-cdc42 signaling regulates BAF complexes and Ewing sarcoma cell growth.Cell reports · 2021Article
- Quantifying the roles of space and stochasticity in computer simulations for cell biology and cellular biochemistry.Molecular biology of the cell · 2021Article
- Layer-Specific Inhibitory Microcircuits of Layer 6 Interneurons in Rat Prefrontal Cortex.Cerebral cortex (New York, N.Y. : 1991) · 2021Article
- Following the footprints of variability during filopodial growth.European biophysics journal : EBJ · 2020Article
- Automated profiling of growth cone heterogeneity defines relations between morphology and motility.The Journal of cell biology · 2019Article
- Filopodyan: An open-source pipeline for the analysis of filopodia.The Journal of cell biology · 2017Article
- The tumor promoter-activated protein kinase Cs are a system for regulating filopodia.Cytoskeleton (Hoboken, N.J.) · 2017Article
- A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions.Journal of visualized experiments : JoVE · 2017Article
- Quantifying Filopodia in Cultured Astrocytes by an Algorithm.Neurochemical research · 2017Article
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9 authors.
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Abstract
Filopodia are dynamic, actin-rich structures that transiently form on a variety of cell types. To understand the underlying control mechanisms requires precise monitoring of localization and concentration of individual regulatory and structural proteins as filopodia elongate and subsequently retract. Although several methods exist that analyze changes in filopodial shape, a software solution to reliably correlate growth dynamics with spatially resolved protein concentration along the filopodium independent of bending, lateral shift, or tilting is missing. Here we introduce a novel approach based on the convex-hull algorithm for parallel analysis of growth dynamics and relative spatiotemporal protein concentration along flexible filopodial protrusions. Detailed in silico tests using various geometries confirm that our technique accurately tracks growth dynamics and relative protein concentration along the filopodial length for a broad range of signal distributions. To validate our technique in living cells, we measure filopodial dynamics and quantify spatiotemporal localization of filopodia-associated proteins during the filopodial extension-retraction cycle in a variety of cell types in vitro and in vivo. Together these results show that the technique is suitable for simultaneous analysis of growth dynamics and spatiotemporal protein enrichment along filopodia. To allow readily application by other laboratories, we share source code and instructions for software handling.
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