Evidence map›Paper›PMID 27535067›Full record

ArticleVirologica Sinica2016

Comparison of concentration methods for detection of hepatitis A virus in water samples.

Yuting Qiao, Zhiwei Sui, Guoliang Hu, Huabin Cao, Guoxiang Yang, Yong Li, Yongsong Lei, Lihua Zhao, Quanjiao Chen

Open access · greenAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Virologica Sinica, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 92% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Yuting QiaoCAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
Zhiwei SuiNational Institute of Metrology, Beijing, 100013, China.
Guoliang HuCollege of Animal Science and Technology, Institute of Animal Population Health, Jiangxi Agricultural University, Nanchang, 330045, China.
Huabin CaoCollege of Animal Science and Technology, Institute of Animal Population Health, Jiangxi Agricultural University, Nanchang, 330045, China.
Guoxiang YangHubei Wildlife Epidemic Focus and Disease Surveillance Center, Wuhan, 430075, China.
Yong LiHubei Wildlife Epidemic Focus and Disease Surveillance Center, Wuhan, 430075, China.
Yongsong LeiHubei Wildlife Epidemic Focus and Disease Surveillance Center, Wuhan, 430075, China.
Lihua ZhaoCAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
Quanjiao ChenCAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. chenqj@wh.iov.cn.
Jiangxi Agricultural University · CNWuhan Institute of Virology · CNNational Institute of Metrology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hepatitis A virus is a pathogen associated with water pollution. Contaminated drinking water can cause hepatitis A outbreaks, lead to economic losses, and even threaten human lives. It is difficult to detect low levels of hepatitis A virus in water, so the virus must be concentrated in order to quantify it accurately. Here, we present a simple, rapid, efficient technique for the concentration and detection of hepatitis A virus in water. Our data showed that adding phosphate-buffered saline to the water, pre-filtering the water, and adding Trizol reagent directly to the filtration membrane can significantly improve concentration efficiency. Of three types of filtration membranes studied (mixed cellulose ester membrane, polyvinylidene fluoride membrane, and nylon membrane), the concentration efficiency using mixed cellulose ester membrane with a 0.1-μm pore size was the highest, reaching 92.62 ± 5.17%. This method was used to concentrate hepatitis A virus in water samples from Donghu Lake. Using SYBR Green real-time reverse transcription polymerase chain reaction analysis, the detection sensitivity of this method reached 10(1) copies/μL and its concentration efficiency reached 79.45 ± 9.88%.

Indexed as

FiltrationFresh WaterHepatitis A virusLakesReal-Time Polymerase Chain ReactionRNA, ViralWater PollutionRNA, Viralconcentrationfiltration membranehepatitis A virusmixed cellulose ester (MCE) membranewater samples

Identifiers

PMID27535067
PMCPMC8193390
OpenAlexW2510894790

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.