ArticleScientific reports2016
Single molecule super-resolution imaging of proteins in living Salmonella enterica using self-labelling enzymes.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 58 citations in OpenAlex.
- An inverse relationship between fitness and secretion efficiency in a gram-positive bacterium.PNAS nexus · 2025Article
- Experimental Approaches to Visualize Effector Protein Translocation During Host-Pathogen Interactions.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Fluorescent labeling strategies for molecular bioimaging.Biophysical reports · 2025Review
- Cell cycle dependent coordination of surface layer biogenesis in Caulobacter crescentus.Nature communications · 2024Article
- Real-time imaging of drug-induced trapping of cellular topoisomerases and poly(ADP-ribose) polymerase 1 at the single-molecule level.Nucleic acids research · 2023Article
- Single molecule analyses reveal dynamics of Salmonella translocated effector proteins in host cell endomembranes.Nature communications · 2023Article
- Quantification and Surface Localization of the Hemolysin A Type I Secretion System at the Endogenous Level and under Conditions of Overexpression.Applied and environmental microbiology · 2022Article
- Recent biomedical advances enabled by HaloTag technology.Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al · 2022Article
- A self-labeling protein based on the small ultra-red fluorescent protein, smURFP.RSC chemical biology · 2021Article
- Versatile Labeling and Detection of Endogenous Proteins Using Tag-Assisted Split Enzyme Complementation.ACS chemical biology · 2021Article
- Bacterial Vivisection: How Fluorescence-Based Imaging Techniques Shed a Light on the Inner Workings of Bacteria.Microbiology and molecular biology reviews : MMBR · 2020Review
- A mathematical model of flagellar gene regulation and construction in Salmonella enterica.PLoS computational biology · 2020Article
- Interrogating surfaceChemical science · 2020Article
- tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging.Royal Society open science · 2019Article
- Advanced Fluorescent Polymer Probes for the Site-Specific Labeling of Proteins in Live Cells Using the HaloTag Technology.ACS omega · 2019Article
- Article
- Quantification of very low-abundant proteins in bacteria using the HaloTag and epi-fluorescence microscopy.Scientific reports · 2019Article
- Single molecule super-resolution imaging of bacterial cell pole proteins with high-throughput quantitative analysis pipeline.Scientific reports · 2019Article
- Choosing the right label for single-molecule tracking in live bacteria: side-by-side comparison of photoactivatable fluorescent protein and Halo tag dyes.Journal of physics D: Applied physics · 2019Article
- ColiCoords: A Python package for the analysis of bacterial fluorescence microscopy data.PloS one · 2019Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The investigation of the subcellular localization, dynamics and interaction of proteins and protein complexes in prokaryotes is complicated by the small size of the cells. Super-resolution microscopy (SRM) comprise various new techniques that allow light microscopy with a resolution that can be up to ten-fold higher than conventional light microscopy. Application of SRM techniques to living prokaryotes demands the introduction of suitable fluorescent probes, usually by fusion of proteins of interest to fluorescent proteins with properties compatible to SRM. Here we describe an approach that is based on the genetically encoded self-labelling enzymes HaloTag and SNAP-tag. Proteins of interest are fused to HaloTag or SNAP-tag and cell permeable substrates can be labelled with various SRM-compatible fluorochromes. Fusions of the enzyme tags to subunits of a type I secretion system (T1SS), a T3SS, the flagellar rotor and a transcription factor were generated and analysed in living Salmonella enterica. The new approach is versatile in tagging proteins of interest in bacterial cells and allows to determine the number, relative subcellular localization and dynamics of protein complexes in living cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.