ArticleScientific reports2016
Identification and characterization of chromosomal relBE toxin-antitoxin locus in Streptomyces cattleya DSM46488.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 18 citations in OpenAlex.
- Degradation Mechanism of AAA+ Proteases and Regulation ofBiomolecules · 2022Review
- Comparative Analysis of Diverse Acetyltransferase-Type Toxin-Antitoxin Loci in Klebsiella pneumoniae.Microbiology spectrum · 2022Article
- Characterization of DinJ-YafQ toxin-antitoxin module in Tetragenococcus halophilus: activity, interplay, and evolution.Applied microbiology and biotechnology · 2021Article
- Antimicrobial Resistance in Veterinary Medicine: An Overview.International journal of molecular sciences · 2020Review
- Characterization of Two Toxin-Antitoxin Systems in Deep-SeaMarine drugs · 2019Article
- Article
- Impacts of horizontal gene transfer on the compact genome of the clavulanic acid-producing3 Biotech · 2018Article
- Identification of a functional toxin-antitoxin system located in the genomic island PYG1 of piezophilic hyperthermophilic archaeon Pyrococcus yayanosii.Extremophiles : life under extreme conditions · 2018Article
- TADB 2.0: an updated database of bacterial type II toxin-antitoxin loci.Nucleic acids research · 2018Article
- Antibiotics, Resistome and Resistance Mechanisms: A Bacterial Perspective.Frontiers in microbiology · 2018Review
- Complete genome sequencing and antibiotics biosynthesis pathways analysis of Streptomyces lydicus 103.Scientific reports · 2017Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The relBE family of Type II toxin-antitoxin (TA) systems have been widely reported in bacteria but none in Streptomyces. With the conserved domain searches for TA pairs in the sequenced Streptomyces genomes, we identified two putative relBE loci, relBE1sca and relBE2sca, on the chromosome of Streptomyces cattleya DSM 46488. Overexpression of the S. cattleya toxin RelE2sca caused severe growth inhibition of E. coli and S. lividans, but RelE1sca had no toxic effect. The toxicity of RelE2sca could be abolished by the co-expression of its cognate RelB2sca antitoxin. Moreover, the RelBE2sca complex, or the antitoxin RelB2sca alone, specifically interacted with the relBE2sca operon and repressed its transcription. The relBE2sca operon transcription was induced under osmotic stress, along with the ClpP proteinase genes. The subsequent in vivo analysis showed that the antitoxin was degraded by ClpP. Interestingly, the E. coli antitoxin RelBeco was able to alleviate the toxicity of S. cattleya RelE2sca while the mutant RelB2sca(N61V&M68L) but not the wild type could alleviate the toxicity of E. coli RelEeco as well. The experimental demonstration of the relBEsca locus might be helpful to investigate the key roles of type II TA systems in Streptomyces physiology and environmental stress responses.
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