Evidence map›Paper›PMID 27534381›Full record

ArticleExperimental gerontology2016

Age-related alterations in the sarcolemmal environment are attenuated by lifelong caloric restriction and voluntary exercise.

Jeffrey M Hord, Rachel Botchlett, John M Lawler

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental gerontology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
0.8field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 31 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Jeffrey M HordRedox Biology & Cell Signaling Laboratory, Department of Health and Kinesiology, College of Education and Human Development, Texas A&M University, College Station, TX, United States.
Rachel BotchlettDepartment of Nutrition & Food Science, College of Agriculture and Life Sciences, Texas A&M University, College Station, TX, United States.
John M LawlerRedox Biology & Cell Signaling Laboratory, Department of Health and Kinesiology, College of Education and Human Development, Texas A&M University, College Station, TX, United States; Department of Nutrition & Food Science, College of Agriculture and Life Sciences, Texas A&M University, College Station, TX, United States. Electronic address: jml2621@tamu.edu.
Texas A&M University · US

Funding

Regulation of Oxidative Stress in the mdx DiaphragmR03AR054084 · NIAMS · TEXAS A&M UNIVERSITY · PI LAWLER, JOHN M · 2006 to 2008
$204k
NIAMS NIH HHS R03 AR054084
6 · The paper itself

Abstract

Age-related loss of skeletal muscle mass and function, referred to as sarcopenia, is mitigated by lifelong calorie restriction as well as exercise. In aged skeletal muscle fibers there is compromised integrity of the cell membrane that may contribute to sarcopenia. The purpose of this study was to determine if lifelong mild (8%) caloric restriction (CR) and lifelong CR+voluntary wheel running (WR) could ameliorate disruption of membrane scaffolding and signaling proteins during the aging process, thus maintaining a favorable, healthy membrane environment in plantaris muscle fibers. Fischer-344 rats were divided into four groups: 24-month old adults fed ad libitum (OAL); 24-month old on 8% caloric restriction (OCR); 24month old 8% caloric restriction+wheel running (OCRWR); and 6-month old sedentary adults fed ad libitum (YAL) were used to determine age-related changes. Aging resulted in discontinuous membrane expression of dystrophin glycoprotein complex (DGC) proteins: dystrophin and α-syntrophin. Older muscle also displayed decreased content of neuronal nitric oxide synthase (nNOS), a key DGC signaling protein. In contrast, OCR and OCRWR provided significant protection against age-related DGC disruption. In conjunction with the age-related decline in membrane DGC patency, key membrane repair proteins (MG53, dysferlin, annexin A6, and annexin A2) were significantly increased in the OAL plantaris. However, lifelong CR and CRWR interventions were effective at maintaining membrane repair proteins near YAL levels of. OAL fibers also displayed reduced protein content of NADPH oxidase isoform 2 (Nox2) subunits (p67phox and p47phox), consistent with a perturbed sarcolemmal environment. Loss of Nox2 subunits was prevented by lifelong CR and CRWR. Our results are therefore consistent with the hypothesis that lifelong CR and WR are effective countermeasures against age-related alterations in the myofiber membrane environment.

Indexed as

Caloric RestrictionAgingAnimalsApoptosisCalcium-Binding ProteinsDystrophinImmunohistochemistryMaleMembrane ProteinsMuscle Fibers, SkeletalMuscle ProteinsOrgan SizeOxidative StressPhysical Conditioning, AnimalRatsRats, Inbred F344Calcium-Binding ProteinsDystrophinMembrane ProteinsMuscle Proteinssyntrophin alpha1Aging muscleCalorie restrictionExerciseSarcolemmaSarcopeniaWheel running

Identifiers

PMID27534381
PMCPMC5026642
OpenAlexW2509514813

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.