ArticleCurrent protocols in stem cell biology2016
Comprehensive Protocols for CRISPR/Cas9-based Gene Editing in Human Pluripotent Stem Cells.
Article in Current protocols in stem cell biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- CRISPR/Cas9-based repair of a heterozygous HNF1A mutation in patient-derived hiPSCs.Human genetics · 2026Article
- Protocol for enhancing CRISPR-Cas9 genome editing using histone deacetylase inhibition and engineered virus-like particle delivery.STAR protocols · 2026Article
- Macrophage surface protein Mac-2 mediates inflammatory and stromal stress pathways in doxorubicin-induced cardiac injury.Cardio-oncology (London, England) · 2026Article
- The ACBD3 protein coordinates ER-Golgi contacts to enable productive TBEV infection.Journal of virology · 2025Article
- ALS-associated C21ORF2 variant disrupts DNA damage repair, mitochondrial metabolism, neuronal excitability and NEK1 levels in human motor neurons.Acta neuropathologica communications · 2024Article
- Article
- Differentiation, Transcriptomic Profiling, and Calcium Imaging of Human Hypothalamic Neurons.Current protocols · 2023Article
- Concise Review: Stem Cell Models ofCells · 2022Review
- Homozygous might be hemizygous: CRISPR/Cas9 editing in iPSCs results in detrimental on-target defects that escape standard quality controls.Stem cell reports · 2022Article
- The efficient generation of knockout microglia cells using a dual-sgRNA strategy by CRISPR/Cas9.Frontiers in molecular neuroscience · 2022Article
- Cardiac cell type-specific gene regulatory programs and disease risk association.Science advances · 2021Article
- Inherited cardiac diseases, pluripotent stem cells, and genome editing combined-the past, present, and future.Stem cells (Dayton, Ohio) · 2020Review
- Pentameric Ligand-Gated Ion Channels as Pharmacological Targets Against Chronic Pain.Frontiers in pharmacology · 2020Review
- Developments in cell culture systems for human pluripotent stem cells.World journal of stem cells · 2019Review
- Transcriptionally active HERV-H retrotransposons demarcate topologically associating domains in human pluripotent stem cells.Nature genetics · 2019Article
- lncRNA PSORS1C3 is regulated by glucocorticoids and fine-tunes OCT4 expression in non-pluripotent cells.Scientific reports · 2019Article
- Functional identity of hypothalamic melanocortin neurons depends on Tbx3.Nature metabolism · 2019Article
- CRISPR/Cas9-based Targeted Genome Editing for the Development of Monogenic Diseases Models with Human Pluripotent Stem Cells.Current protocols in stem cell biology · 2018Article
- A Scaled Framework for CRISPR Editing of Human Pluripotent Stem Cells to Study Psychiatric Disease.Stem cell reports · 2017Article
- Editing the genome of hiPSC with CRISPR/Cas9: disease models.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Genome editing of human pluripotent stem cells (hPSCs) with the CRISPR/Cas9 system has the potential to revolutionize hPSC-based disease modeling, drug screening, and transplantation therapy. Here, we aim to provide a single resource to enable groups, even those with limited experience with hPSC culture or the CRISPR/Cas9 system, to successfully perform genome editing. The methods are presented in detail and are supported by a theoretical framework to allow for the incorporation of inevitable improvements in the rapidly evolving gene-editing field. We describe protocols to generate hPSC lines with gene-specific knock-outs, small targeted mutations, or knock-in reporters. © 2016 by John Wiley & Sons, Inc.
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Registered trials
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