ArticlePloS one2016
Exposure of Lactating Dairy Cows to Acute Pre-Ovulatory Heat Stress Affects Granulosa Cell-Specific Gene Expression Profiles in Dominant Follicles.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 21 citations in OpenAlex.
- The effects of heat stress on the ovary, follicles, and oocytes: a systematic review†.Biology of reproduction · 2025Pooled it
- Effects of extending interval between presynchronization and breeding protocols and prostaglandin F2α administration before breeding protocol on reproductive performance of Holstein dairy cows during summer.Veterinary and animal science · 2026Article
- Ovarian Function, Hormonal, Metabolic, Oxidative Stress, and Gene Expression Biomarker Responses of Holstein Heifers Under Heat Stress Conditions to a Reduced Dose of Nanofabricated GnRH Analog Within the Ovsynch Protocol.Animals : an open access journal from MDPI · 2026Article
- Investigating ovarian effects of perfluorooctanoic acid in postpubertal pigs during thermal neutral and heat stress conditions.Journal of animal science · 2026Article
- Six things to know about how heat stress compromises fertility of the cow (and probably other mammals as well).The Journal of reproduction and development · 2026Review
- Impact of Heat Stress on Ovarian Function and circRNA Expression in Hu Sheep.Animals : an open access journal from MDPI · 2025Article
- Advances in the Effects of Heat Stress on Ovarian Granulosa Cells: Unveiling Novel Ferroptosis Pathways.Veterinary sciences · 2024Review
- Impact of Heat Stress on Oocyte Developmental Competence and Pre-Implantation Embryo Viability in Cattle.Animals : an open access journal from MDPI · 2024Review
- Heat Stress: A Serious Disruptor of the Reproductive Physiology of Dairy Cows.Animals : an open access journal from MDPI · 2023Review
- Developmental, cytogenetic and epigenetic consequences of removing complex proteins and adding melatonin during in vitro maturation of bovine oocytes.Frontiers in endocrinology · 2023Article
- Gene Expression of Aquaporins (AQPs) in Cumulus Oocytes Complex and Embryo of Cattle.Animals : an open access journal from MDPI · 2022Article
- Validation of SNP markers for thermotolerance adaptation in Ovis aries adapted to different climatic regions using KASP-PCR technique.Scientific reports · 2022Article
- Heat-induced increases in body temperature in lactating dairy cows: impact on the cumulus and granulosa cell transcriptome of the periovulatory follicle.Journal of animal science · 2022Article
- Effects of Heat Stress on Follicular Physiology in Dairy Cows.Animals : an open access journal from MDPI · 2021Review
- Identification of Reference Genes for Expression Studies in the Whole-Blood from Three Cattle Breeds under Two States of Livestock Weather Safety.Animals : an open access journal from MDPI · 2021Article
- Relation of the maximum temperature and relative humidity close to the insemination with the tropical milking criollo heifer's gestation in three seasons.Tropical animal health and production · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High environmental temperatures induce detrimental effects on various reproductive processes in cattle. According to the predicted global warming the number of days with unfavorable ambient temperatures will further increase. The objective of this study was to investigate effects of acute heat stress during the late pre-ovulatory phase on morphological, physiological and molecular parameters of dominant follicles in cycling cows during lactation. Eight German Holstein cows in established lactation were exposed to heat stress (28°C) or thermoneutral conditions (15°C) with pair-feeding for four days. After hormonal heat induction growth of the respective dominant follicles was monitored by ultrasonography for two days, then an ovulatory GnRH dose was given and follicular steroid hormones and granulosa cell-specific gene expression profiles were determined 23 hrs thereafter. The data showed that the pre-ovulatory growth of dominant follicles and the estradiol, but not the progesterone concentrations tended to be slightly affected. mRNA microarray and hierarchical cluster analysis revealed distinct expression profiles in granulosa cells derived from heat stressed compared to pair-fed animals. Among the 255 affected genes heatstress-, stress- or apoptosis associated genes were not present. But instead, we found up-regulation of genes essentially involved in G-protein coupled signaling pathways, extracellular matrix composition, and several members of the solute carrier family as well as up-regulation of FST encoding follistatin. In summary, the data of the present study show that acute pre-ovulatory heat stress can specifically alter gene expression profiles in granulosa cells, however without inducing stress related genes and pathways and suggestively can impair follicular growth due to affecting the activin-inhibin-follistatin system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.