ArticleProceedings of the National Academy of Sciences of the United States of America2016
Structural conservation in the template/pseudoknot domain of vertebrate telomerase RNA from teleost fish to human.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 27 citations in OpenAlex.
- Metamorphosis and lncRNAs: A Close Relationship.Genesis (New York, N.Y. : 2000) · 2026Review
- Identification of the putative gene encoding the RNA subunit of telomerase in Malassezia clade species through comparative genomic analysis.G3 (Bethesda, Md.) · 2025Article
- Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex.Plant molecular biology · 2024Article
- Linking folding dynamics and function of SAM/SAH riboswitches at the single molecule level.Nucleic acids research · 2023Article
- Structure of LARP7 Protein p65-telomerase RNA Complex in Telomerase Revealed by Cryo-EM and NMR.Journal of molecular biology · 2023Article
- Long non-coding RNAs: definitions, functions, challenges and recommendations.Nature reviews. Molecular cell biology · 2023Review
- Development of RNA G-quadruplex (rG4)-targeting L-RNA aptamers by rG4-SELEX.Nature protocols · 2022Review
- From genotype to phenotype: genetics of mammalian long non-coding RNAs in vivo.Nature reviews. Genetics · 2022Review
- Article
- Structural biology of human telomerase: progress and prospects.Biochemical Society transactions · 2021Review
- RNAs as Regulators of Cellular Matchmaking.Frontiers in molecular biosciences · 2021Review
- A structurally conserved human andProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Structural biology of telomeres and telomerase.Cellular and molecular life sciences : CMLS · 2020Review
- Structural Biology of Telomerase.Cold Spring Harbor perspectives in biology · 2019Review
- Telomerase structures and regulation: shedding light on the chromosome end.Current opinion in structural biology · 2019Review
- Mechanisms of template handling and pseudoknot folding in human telomerase and their manipulation to expand the sequence repertoire of processive repeat synthesis.Nucleic acids research · 2018Article
- Structure of Telomerase with Telomeric DNA.Cell · 2018Article
- Article
- New perspectives on telomerase RNA structure and function.Wiley interdisciplinary reviews. RNA · 2018Review
- Structural biology of telomerase and its interaction at telomeres.Current opinion in structural biology · 2017Review
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Authors and funding
5 authors at 3 institutions in 1 country.
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Abstract
Telomerase is an RNA-protein complex that includes a unique reverse transcriptase that catalyzes the addition of single-stranded telomere DNA repeats onto the 3' ends of linear chromosomes using an integral telomerase RNA (TR) template. Vertebrate TR contains the template/pseudoknot (t/PK) and CR4/5 domains required for telomerase activity in vitro. All vertebrate pseudoknots include two subdomains: P2ab (helices P2a and P2b with a 5/6-nt internal loop) and the minimal pseudoknot (P2b-P3 and associated loops). A helical extension of P2a, P2a.1, is specific to mammalian TR. Using NMR, we investigated the structures of the full-length TR pseudoknot and isolated subdomains in Oryzias latipes (Japanese medaka fish), which has the smallest vertebrate TR identified to date. We determined the solution NMR structure and studied the dynamics of medaka P2ab, and identified all base pairs and tertiary interactions in the minimal pseudoknot. Despite differences in length and sequence, the structure of medaka P2ab is more similar to human P2ab than predicted, and the medaka minimal pseudoknot has the same tertiary interactions as the human pseudoknot. Significantly, although P2a.1 is not predicted to form in teleost fish, we find that it forms in the full-length pseudoknot via an unexpected hairpin. Model structures of the subdomains are combined to generate a model of t/PK. These results provide evidence that the architecture for the vertebrate t/PK is conserved from teleost fish to human. The organization of the t/PK on telomerase reverse transcriptase for medaka and human is modeled based on the cryoEM structure of Tetrahymena telomerase, providing insight into function.
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