Evidence map›Paper›PMID 27531442›Full record

ArticlePlant physiology2016

A Distinct Pathway for Polar Exocytosis in Plant Cell Wall Formation.

Hao Wang, Xiaohong Zhuang, Xiangfeng Wang, Angus Ho Yin Law, Teng Zhao, Shengwang Du, Michael M T Loy, Liwen Jiang

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
4.7field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 50 citations in OpenAlex.

  1. Article
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  5. Quantifying Protein Dynamics by Kymograph Analysis.Methods in molecular biology (Clifton, N.J.) · 2024
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  8. Review
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  11. Article
  12. Review
  13. Review
  14. Article
  15. Article
  16. Visualization of Protein Sorting at theFrontiers in cell and developmental biology · 2019
    Article
  17. Golgi-localized LOT regulatesProceedings of the National Academy of Sciences of the United States of America · 2018
    Article
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Hao WangSchool of Life Sciences, Centre for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China (H.W., X.Z., X.W., A.H.Y.L., L.J.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (H.W.);Department of Physics, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China (T.Z., S.D., M.M.T.L.);Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China (S.D.); andCUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen 518057, China (L.J.).
Xiaohong ZhuangSchool of Life Sciences, Centre for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China (H.W., X.Z., X.W., A.H.Y.L., L.J.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (H.W.);Department of Physics, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China (T.Z., S.D., M.M.T.L.);Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China (S.D.); andCUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen 518057, China (L.J.).
Xiangfeng WangSchool of Life Sciences, Centre for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China (H.W., X.Z., X.W., A.H.Y.L., L.J.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (H.W.);Department of Physics, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China (T.Z., S.D., M.M.T.L.);Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China (S.D.); andCUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen 518057, China (L.J.).
Angus Ho Yin LawSchool of Life Sciences, Centre for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China (H.W., X.Z., X.W., A.H.Y.L., L.J.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (H.W.);Department of Physics, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China (T.Z., S.D., M.M.T.L.);Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China (S.D.); andCUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen 518057, China (L.J.).
Teng ZhaoSchool of Life Sciences, Centre for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China (H.W., X.Z., X.W., A.H.Y.L., L.J.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (H.W.);Department of Physics, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China (T.Z., S.D., M.M.T.L.);Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China (S.D.); andCUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen 518057, China (L.J.).
Shengwang DuSchool of Life Sciences, Centre for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China (H.W., X.Z., X.W., A.H.Y.L., L.J.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (H.W.);Department of Physics, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China (T.Z., S.D., M.M.T.L.);Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China (S.D.); andCUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen 518057, China (L.J.).
Michael M T LoySchool of Life Sciences, Centre for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China (H.W., X.Z., X.W., A.H.Y.L., L.J.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (H.W.);Department of Physics, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China (T.Z., S.D., M.M.T.L.);Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China (S.D.); andCUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen 518057, China (L.J.).
Liwen JiangSchool of Life Sciences, Centre for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China (H.W., X.Z., X.W., A.H.Y.L., L.J.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (H.W.);Department of Physics, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China (T.Z., S.D., M.M.T.L.);Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China (S.D.); andCUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen 518057, China (L.J.) ljiang@cuhk.edu.hk.
Chinese University of Hong Kong · CNUniversity of Hong Kong · HKChinese University of Hong Kong, Shenzhen · CNHong Kong University of Science and Technology · HK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Post-Golgi protein sorting and trafficking to the plasma membrane (PM) is generally believed to occur via the trans-Golgi network (TGN). In this study using Nicotiana tabacum pectin methylesterase (NtPPME1) as a marker, we have identified a TGN-independent polar exocytosis pathway that mediates cell wall formation during cell expansion and cytokinesis. Confocal immunofluorescence and immunogold electron microscopy studies demonstrated that Golgi-derived secretory vesicles (GDSVs) labeled by NtPPME1-GFP are distinct from those organelles belonging to the conventional post-Golgi exocytosis pathway. In addition, pharmaceutical treatments, superresolution imaging, and dynamic studies suggest that NtPPME1 follows a polar exocytic process from Golgi-GDSV-PM/cell plate (CP), which is distinct from the conventional Golgi-TGN-PM/CP secretion pathway. Further studies show that ROP1 regulates this specific polar exocytic pathway. Taken together, we have demonstrated an alternative TGN-independent Golgi-to-PM polar exocytic route, which mediates secretion of NtPPME1 for cell wall formation during cell expansion and cytokinesis and is ROP1-dependent.

Indexed as

ExocytosisAntioxidantsCarboxylic Ester HydrolasesCatechinCell DivisionCell LineCell MembraneCell WallCytokinesisGreen Fluorescent ProteinsMicroscopy, ConfocalMicroscopy, ImmunoelectronNicotianaPlant ProteinsPollenProtein TransportAntioxidantsCarboxylic Ester HydrolasesCatechinepigallocatechin gallateGreen Fluorescent ProteinspectinesterasePlant Proteins

Identifiers

PMID27531442
PMCPMC5047090
OpenAlexW2528598188

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.