Evidence map›Paper›PMID 27531441›Full record

ArticlePlant physiology2016

In Planta Localization of Stilbenes within Picea abies Phloem.

Tuula Jyske, Katsushi Kuroda, Jussi-Petteri Suuronen, Andrey Pranovich, Sílvia Roig-Juan, Dan Aoki, Kazuhiko Fukushima

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Localization of (+)-Catechin inMolecules (Basel, Switzerland) · 2020
    Article
  5. Review
  6. Article
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 5 institutions in 3 countries.

Tuula JyskeNatural Resources Institute Finland, New Business Opportunities, 01370 Vantaa, Finland (T.J., S.R.-J.); Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba 305-8687, Japan (K.K.);University of Helsinki, Department of Physics, 00014 Helsinki, Finland (J.-P.S.);European Synchrotron Radiation Facility, 38043 Grenoble cedex 9, France (J.-P.S.);Åbo Akademi University, Johan Gadolin Process Chemistry Center, 20500 Turku, Finland (A.P.); andNagoya University, Department of Biosphere Resources Science, Chikusa-ku, Nagoya 464-8601, Japan (D.A., K.F.) tuula.jyske@luke.fi.ORCID 0000-0002-0459-4358
Katsushi KurodaNatural Resources Institute Finland, New Business Opportunities, 01370 Vantaa, Finland (T.J., S.R.-J.); Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba 305-8687, Japan (K.K.);University of Helsinki, Department of Physics, 00014 Helsinki, Finland (J.-P.S.);European Synchrotron Radiation Facility, 38043 Grenoble cedex 9, France (J.-P.S.);Åbo Akademi University, Johan Gadolin Process Chemistry Center, 20500 Turku, Finland (A.P.); andNagoya University, Department of Biosphere Resources Science, Chikusa-ku, Nagoya 464-8601, Japan (D.A., K.F.).
Jussi-Petteri SuuronenNatural Resources Institute Finland, New Business Opportunities, 01370 Vantaa, Finland (T.J., S.R.-J.); Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba 305-8687, Japan (K.K.);University of Helsinki, Department of Physics, 00014 Helsinki, Finland (J.-P.S.);European Synchrotron Radiation Facility, 38043 Grenoble cedex 9, France (J.-P.S.);Åbo Akademi University, Johan Gadolin Process Chemistry Center, 20500 Turku, Finland (A.P.); andNagoya University, Department of Biosphere Resources Science, Chikusa-ku, Nagoya 464-8601, Japan (D.A., K.F.).ORCID 0000-0002-1632-761X
Andrey PranovichNatural Resources Institute Finland, New Business Opportunities, 01370 Vantaa, Finland (T.J., S.R.-J.); Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba 305-8687, Japan (K.K.);University of Helsinki, Department of Physics, 00014 Helsinki, Finland (J.-P.S.);European Synchrotron Radiation Facility, 38043 Grenoble cedex 9, France (J.-P.S.);Åbo Akademi University, Johan Gadolin Process Chemistry Center, 20500 Turku, Finland (A.P.); andNagoya University, Department of Biosphere Resources Science, Chikusa-ku, Nagoya 464-8601, Japan (D.A., K.F.).
Sílvia Roig-JuanNatural Resources Institute Finland, New Business Opportunities, 01370 Vantaa, Finland (T.J., S.R.-J.); Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba 305-8687, Japan (K.K.);University of Helsinki, Department of Physics, 00014 Helsinki, Finland (J.-P.S.);European Synchrotron Radiation Facility, 38043 Grenoble cedex 9, France (J.-P.S.);Åbo Akademi University, Johan Gadolin Process Chemistry Center, 20500 Turku, Finland (A.P.); andNagoya University, Department of Biosphere Resources Science, Chikusa-ku, Nagoya 464-8601, Japan (D.A., K.F.).
Dan AokiNatural Resources Institute Finland, New Business Opportunities, 01370 Vantaa, Finland (T.J., S.R.-J.); Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba 305-8687, Japan (K.K.);University of Helsinki, Department of Physics, 00014 Helsinki, Finland (J.-P.S.);European Synchrotron Radiation Facility, 38043 Grenoble cedex 9, France (J.-P.S.);Åbo Akademi University, Johan Gadolin Process Chemistry Center, 20500 Turku, Finland (A.P.); andNagoya University, Department of Biosphere Resources Science, Chikusa-ku, Nagoya 464-8601, Japan (D.A., K.F.).
Kazuhiko FukushimaNatural Resources Institute Finland, New Business Opportunities, 01370 Vantaa, Finland (T.J., S.R.-J.); Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba 305-8687, Japan (K.K.);University of Helsinki, Department of Physics, 00014 Helsinki, Finland (J.-P.S.);European Synchrotron Radiation Facility, 38043 Grenoble cedex 9, France (J.-P.S.);Åbo Akademi University, Johan Gadolin Process Chemistry Center, 20500 Turku, Finland (A.P.); andNagoya University, Department of Biosphere Resources Science, Chikusa-ku, Nagoya 464-8601, Japan (D.A., K.F.).ORCID 0000-0001-8651-2733
Nagoya University · JPNatural Resources Institute Finland · FIÅbo Akademi University · FIForestry and Forest Products Research Institute · JPHelsinki Institute of Physics · FI

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Phenolic stilbene glucosides (astringin, isorhapontin, and piceid) and their aglycons commonly accumulate in the phloem of Norway spruce (Picea abies). However, current knowledge about the localization and accumulation of stilbenes within plant tissues and cells remains limited. Here, we used an innovative combination of novel microanalytical techniques to evaluate stilbenes in a frozen-hydrated condition (i.e. in planta) and a freeze-dried condition across phloem tissues. Semiquantitative time-of-flight secondary ion-mass spectrometry imaging in planta revealed that stilbenes were localized in axial parenchyma cells. Quantitative gas chromatography analysis showed the highest stilbene content in the middle of collapsed phloem with decreases toward the outer phloem. The same trend was detected for soluble sugar and water contents. The specimen water content may affect stilbene composition; the glucoside-to-aglycon ratio decreased slightly with decreases in water content. Phloem chemistry was correlated with three-dimensional structures of phloem as analyzed by microtomography. The outer phloem was characterized by a high volume of empty parenchyma, reduced ray volume, and a large number of axial parenchyma with porous vacuolar contents. Increasing porosity from the inner to the outer phloem was related to decreasing compactness of stilbenes and possible secondary oxidation or polymerization. Our results indicate that aging-dependent changes in phloem may reduce cell functioning, which affects the capacity of the phloem to store water and sugar, and may reduce the defense potential of stilbenes in the axial parenchyma. Our results highlight the power of using a combination of techniques to evaluate tissue- and cell-level mechanisms involved in plant secondary metabolite formation and metabolism.

Indexed as

Freeze DryingGas Chromatography-Mass SpectrometryGlucosidesImaging, Three-DimensionalMicroscopy, Electron, ScanningPhloemPiceaSpectrometry, Mass, Secondary IonStilbenesWaterX-Ray MicrotomographyastringinGlucosidesisorhapontinpolydatinStilbenesWater

Identifiers

PMID27531441
PMCPMC5047067
OpenAlexW2515090274

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.