Evidence map›Paper›PMID 27531133›Full record

ArticleFish physiology and biochemistry2017

Nucleic acids digestion by enzymes in the stomach of snakehead (Channa argus) and banded grouper (Epinephelus awoara).

Yu Liu, Yanfang Zhang, Wei Jiang, Jing Wang, Xiaoming Pan, Wei Wu, Minjie Cao, Ping Dong, Xingguo Liang

Abstract read
PubMed Publisher
In one paragraph

Article in Fish physiology and biochemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.1field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Yu LiuCollege of Food Science and Engineering, Ocean University of China, Qingdao, China.
Yanfang ZhangCollege of Food Science and Engineering, Ocean University of China, Qingdao, China.
Wei JiangInnovation and Application Institute, Zhejiang Ocean University, Zhoushan, 316022, China.
Jing WangCollege of Food Science and Engineering, Ocean University of China, Qingdao, China.
Xiaoming PanCollege of Food Science and Engineering, Ocean University of China, Qingdao, China.
Wei WuCollege of Food Science and Engineering, Ocean University of China, Qingdao, China.
Minjie CaoCollege of Biological Engineering, Jimei University, Xiamen, China.
Ping DongCollege of Food Science and Engineering, Ocean University of China, Qingdao, China. dongping@ouc.edu.cn.
Xingguo LiangCollege of Food Science and Engineering, Ocean University of China, Qingdao, China. liangxg@ouc.edu.cn.
Ocean University of China · CNJimei University · CNZhejiang Ocean University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Dietary nucleic acids (NAs) were important nutrients. However, the digestion of NAs in stomach has not been studied. In this study, the digestion of NAs by enzymes from fish stomach was investigated. The snakehead pepsins (SP) which were the main enzymes in stomach were extracted and purified. The purity of SP was evaluated by SDS-PAGE and HPLC. The snakehead pepsin 2 (SP2) which was the main component in the extracts was used for investigating the protein and NAs digestion activity. SP2 could digest NAs, including λ DNA and salmon sperm DNA. Interestingly, the digestion could be inhibited by treatment of alkaline solution at pH 8.0 and pepstatin A, and the digestion could happen either in the presence or absence of hemoglobin (Hb) and BSA as the protein substrates. Similarly, the stomach enzymes of banded grouper also showed the NAs digestion activity. NAs could be digested by the stomach enzymes of snakehead and banded grouper. It may be helpful for understanding both animal nutrition and NAs metabolic pathway.

Indexed as

Animal Nutritional Physiological PhenomenaAnimalsCattleDigestionDNAFish ProteinsHemoglobinsPepsin APepstatinsPerciformesSerum Albumin, BovineStomachDNAFish ProteinsHemoglobinsPepsin ApepstatinPepstatinsSerum Albumin, BovineExternal nucleic acidsFeedFishPepsinStomach

Identifiers

PMID27531133
OpenAlexW2516483454

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.