ArticleScientific reports2016
Efficient Genome Editing in Apple Using a CRISPR/Cas9 system.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 142 papers.
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Who cites it
142 citing papers in PubMed, 342 citations in OpenAlex.
- Molecular insights and integrated management of Glomerella leaf spot in apple.Horticulture research · 2026Review
- Article
- Targeting a conserved functional motif in the PDS gene enables efficient CRISPR/Cas9 editing in banana.Scientific reports · 2026Article
- CRISPR/Cas9-based gene-editing platform development by targeting genes encoding magnesium chelatase and phytoene desaturase in sugarbeet (Frontiers in genome editing · 2026Article
- Nanotechnology Strategies in Plant Genetic Engineering: Intelligent Delivery and Precision Editing.Plants (Basel, Switzerland) · 2025Review
- Walnut genome editing: an optimized CRISPR/Cas9 platform with superior genotypes and endogenous promoters.Horticulture research · 2025Article
- CRISPR/Cas-mediated genome editing: playing a versatile role in mitigating the challenges of sustainable rice improvement.3 Biotech · 2025Review
- Multiplex CRISPR-Cas9 editing of chlorophyll biosynthesis genes in chickpea via protoplast and Agrobacterium-mediated transformation.Functional & integrative genomics · 2025Article
- A Truncated Endogenous U6 Promoter Enables High-Efficiency CRISPR Editing in Flax (Plants (Basel, Switzerland) · 2025Article
- Highly Efficient Homozygous CRISPR/Cas9 Gene Editing Based on Single-Cell-Originated Somatic Embryogenesis inPlants (Basel, Switzerland) · 2025Article
- CRISPR-based gene editing in plants: Focus on reagents and their delivery tools.BioImpacts : BI · 2025Review
- Efficient CRISPR/Frontiers in plant science · 2025Article
- From data to decisions: a paradigm shift in fruit agriculture through the integration of multi-omics, modern phenotyping, and cutting-edge bioinformatic tools.Frontiers in plant science · 2025Review
- Identification of U6 Promoter and Establishment of Gene-Editing System inPlants (Basel, Switzerland) · 2024Article
- Genome editing in cells of apple cultivar 'Fuji' using geminivirus-derived replicons for transient expression of CRISPR/Cas9 components.Plant biotechnology (Tokyo, Japan) · 2024Article
- Efficient gene editing of a model fern species through gametophyte-based transformation.Plant physiology · 2024Article
- High-temperature stress in strawberry: understanding physiological, biochemical and molecular responses.Planta · 2024Review
- Phenotyping, genetics, and "-omics" approaches to unravel and introgress enhanced resistance against apple scab (Horticulture research · 2024Article
- Precision Genome Editing Techniques in Gene Therapy: Current State and Future Prospects.Current gene therapy · 2024Review
- Genetic and physiological characteristics ofFrontiers in genome editing · 2024Article
82 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome editing is a powerful technique for genome modification in molecular research and crop breeding, and has the great advantage of imparting novel desired traits to genetic resources. However, the genome editing of fruit tree plantlets remains to be established. In this study, we describe induction of a targeted gene mutation in the endogenous apple phytoene desaturase (PDS) gene using the CRISPR/Cas9 system. Four guide RNAs (gRNAs) were designed and stably transformed with Cas9 separately in apple. Clear and partial albino phenotypes were observed in 31.8% of regenerated plantlets for one gRNA, and bi-allelic mutations in apple PDS were confirmed by DNA sequencing. In addition, an 18-bp gRNA also induced a targeted mutation. These CRIPSR/Cas9 induced-mutations in the apple genome suggest activation of the NHEJ pathway, but with some involvement also of the HR pathway. Our results demonstrate that genome editing can be practically applied to modify the apple genome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.