Evidence map›Paper›PMID 27530693›Full record

ArticleNeurocritical care2017

Post-ischemic Intravenous Administration of Allogeneic Dental Pulp-Derived Neurosphere Cells Ameliorated Outcomes of Severe Forebrain Ischemia in Rats.

Airi Kumasaka, Kaoru Kanazawa, Hanako Ohke, Ikumi Miura, Yoshihide Miura

Abstract read
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Article in Neurocritical care, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Recent Advances in Cell-Based Therapies for Ischemic Stroke.International journal of molecular sciences · 2020
    Review
  3. Review
  4. Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Airi KumasakaDepartment of Anesthesiology, Yamagata University School of Medicine, Yamagata, Japan.
Kaoru KanazawaDepartment of Dental Anesthesiology, School of Dentistry, Health Sciences University of Hokkaido, Kanazawa, Japan.
Hanako OhkeDepartment of Dental Anesthesiology, School of Dentistry, Health Sciences University of Hokkaido, Kanazawa, Japan.
Ikumi MiuraSchool of Human Science and Environment, University of Hyogo, Kobe, Japan.
Yoshihide MiuraDepartment of Dental Anesthesiology, School of Dentistry, Health Sciences University of Hokkaido, Kanazawa, Japan. ymiura@hoku-iryo-u.ac.jp.ORCID 0000-0003-1512-5028

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundTransplantation of bone marrow or adipose-derived mesenchymal stem cells (MSCs) for various neurological disorders has yielded promising results in models of focal cerebral ischemia. Dental pulp stem cells (DPSCs) are a type of MSC. In serum-free culture, they can form neurospheres that contain nestin-positive neuronal progenitor cells. We hypothesized that transplantation of dental pulp-derived neurosphere cells would ameliorate outcomes of global cerebral ischemia, the pathophysiology of which is known to resist conventional treatments. We also hypothesized that transplantation of dental pulp-derived cells would provide some neuroprotection in this pathology due to the presence of DPSCs.

methodsUsing adult rats, ischemia was induced by two-vessel occlusion of both carotid arteries in combination with systemic hypotension. Allogeneic dental pulp cells from juvenile rats were cultured in advance in serum-free medium to obtain neurospheres. Dental pulp-derived neurosphere cells or dental pulp-derived cells were intravenously administered at 3 h after ischemic insult, with normal saline as a control. Animals were observed for 14 days after ischemia. Neurological outcome was assessed using the water-maze test and neuromotor test. Histological outcome was measured by counting the percentage of dead neurons in the hippocampal CA1 and CA3 regions.

resultsTransplantation of both dental pulp-derived neurosphere cells and dental pulp-derived cells significantly improved survival rate and water-maze test results. Neurosphere cell transplantation was related to significantly better neuromotor test and histological outcomes, as indicated by the reduced percentage of dead neurons in CA1.

conclusionsTransplantation of dental pulp-derived neurosphere cells ameliorated outcomes of global cerebral ischemia. It was also demonstrated that dental pulp-derived cell administration provided some neuroprotection.

Indexed as

Dental PulpAdministration, IntravenousAnimalsBrain IschemiaMaleMesenchymal Stem Cell TransplantationNeural Stem CellsProsencephalonRatsRats, Sprague-DawleyDental pulpDental pulp stem cellsGlobal cerebral ischemiaNeurosphereRat

Identifiers

PMID27530693

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.