ArticleScientific reports2016
CRISPR-PCS: a powerful new approach to inducing multiple chromosome splitting in Saccharomyces cerevisiae.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 39 citations in OpenAlex.
- Synthetic Chromosomes in Yeast: A Platform for Understanding and Programming Eukaryotic Life.Microbial biotechnology · 2026Review
- Enhancing Crop Resilience to Drought Stress through CRISPR-Cas9 Genome Editing.Plants (Basel, Switzerland) · 2023Review
- Transcription activator-like effector nuclease (TALEN) as a promising diagnostic approach for COVID-19.Expert review of molecular diagnostics · 2022Article
- COVID-19: A review of newly formed viral clades, pathophysiology, therapeutic strategies and current vaccination tasks.International journal of biological macromolecules · 2021Review
- CRISPR-Cas9-based genetic engineering for crop improvement under drought stress.Bioengineered · 2021Review
- Recent Advances in Genome Editing Tools in Medical Mycology Research.Journal of fungi (Basel, Switzerland) · 2021Review
- Systematic approach for assessing whether undeletable chromosomal regions in Saccharomyces cerevisiae are required for cell viability.AMB Express · 2020Article
- CRISPR-PCDup: a novel approach for simultaneous segmental chromosomal duplication in Saccharomyces cerevisiae.AMB Express · 2020Article
- CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner.Scientific reports · 2019Article
- An extra copy of the β-glucosidase gene improved the cellobiose fermentation capability of an engineered3 Biotech · 2019Article
- Reshuffling yeast chromosomes with CRISPR/Cas9.PLoS genetics · 2019Article
- Improved bioethanol production using CRISPR/Cas9 to disrupt the ADH2 gene in Saccharomyces cerevisiae.World journal of microbiology & biotechnology · 2018Article
- Tuning CRISPR-Cas9 Gene Drives inG3 (Bethesda, Md.) · 2018Article
- The Role of Gene Editing in Neurodegenerative Diseases.Cell transplantation · 2018Review
- Yeast Still a Beast: Diverse Applications of CRISPR/Cas Editing Technology inThe Yale journal of biology and medicine · 2017Review
- Method for Multiplexing CRISPR/Cas9 inBio-protocol · 2017Article
- CRISPR-UnLOCK: Multipurpose Cas9-Based Strategies for Conversion of Yeast Libraries and Strains.Frontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PCR-mediated chromosome splitting (PCS) was developed in the yeast Saccharomyces cerevisiae. It is based on homologous recombination and enables division of a chromosome at any point to form two derived and functional chromosomes. However, because of low homologous recombination activity, PCS is limited to a single site at a time, which makes the splitting of multiple loci laborious and time-consuming. Here we have developed a highly efficient and versatile chromosome engineering technology named CRISPR-PCS that integrates PCS with the novel genome editing CRISPR/Cas9 system. This integration allows PCS to utilize induced double strand breaks to activate homologous recombination. CRISPR-PCS enhances the efficiency of chromosome splitting approximately 200-fold and enables generation of simultaneous multiple chromosome splits. We propose that CRISPR-PCS will be a powerful tool for breeding novel yeast strains with desirable traits for specific industrial applications and for investigating genome function.
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