ArticleNucleic acids research2016
Structure of a human pre-40S particle points to a role for RACK1 in the final steps of 18S rRNA processing.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 49 citations in OpenAlex.
- Nucleoplasmic checkpoint of the 40S ribosomal decoding center maturation.Cell reports · 2026Article
- Mapping human pre-rRNA processing and modification at single nucleotide resolution using long read nanopore sequencing.Nature communications · 2026Article
- The Beak of Eukaryotic Ribosomes: Life, Work and Miracles.Biomolecules · 2024Review
- Ribosome Biogenesis and Cancer: Insights into NOB1 and PNO1 Mechanisms.Current pharmaceutical design · 2024Review
- Ribosome biogenesis factors-from names to functions.The EMBO journal · 2023Review
- Neuronal ribosomes exhibit dynamic and context-dependent exchange of ribosomal proteins.Nature communications · 2021Article
- Efficient fractionation and analysis of ribosome assembly intermediates in human cells.RNA biology · 2021Article
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- A memory of eS25 loss drives resistance phenotypes.Nucleic acids research · 2020Article
- Structural snapshots of human pre-60S ribosomal particles before and after nuclear export.Nature communications · 2020Article
- USP16 counteracts mono-ubiquitination of RPS27a and promotes maturation of the 40S ribosomal subunit.eLife · 2020Article
- Good Vibrations: Structural Remodeling of Maturing Yeast Pre-40S Ribosomal Particles Followed by Cryo-Electron Microscopy.Molecules (Basel, Switzerland) · 2020Article
- Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis.Nature communications · 2020Article
- In vitro dimerization of human RIO2 kinase.RNA biology · 2019Article
- nPKCε Mediates SNAP-25 Phosphorylation of Ser-187 in Basal Conditions and After Synaptic Activity at the Neuromuscular Junction.Molecular neurobiology · 2019Article
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- RACK1 on and off the ribosome.RNA (New York, N.Y.) · 2019Article
- Uncovering the assembly pathway of human ribosomes and its emerging links to disease.The EMBO journal · 2019Review
- Conformational proofreading of distant 40S ribosomal subunit maturation events by a long-range communication mechanism.Nature communications · 2019Article
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Authors and funding
6 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synthesis of ribosomal subunits in eukaryotes is a complex and tightly regulated process that has been mostly characterized in yeast. The discovery of a growing number of diseases linked to defects in ribosome biogenesis calls for a deeper understanding of these mechanisms and of the specificities of human ribosome maturation. We present the 19 Å resolution cryo-EM reconstruction of a cytoplasmic precursor to the human small ribosomal subunit, purified by using the tagged ribosome biogenesis factor LTV1 as bait. Compared to yeast pre-40S particles, this first three-dimensional structure of a human 40S subunit precursor shows noticeable differences with respect to the position of ribosome biogenesis factors and uncovers the early deposition of the ribosomal protein RACK1 during subunit maturation. Consistently, RACK1 is required for efficient processing of the 18S rRNA 3'-end, which might be related to its role in translation initiation. This first structural analysis of a human pre-ribosomal particle sets the grounds for high-resolution studies of conformational transitions accompanying ribosomal subunit maturation.
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