ArticleNucleic acids research2016
Selective single cell isolation for genomics using microraft arrays.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Development of large-scale gastruloid array to identify aberrant developmental phenotypes.APL bioengineering · 2025Article
- Integrative machine learning approach for forecasting lung cancer chemosensitivity: From algorithm to cell line validation.Computational and structural biotechnology journal · 2025Article
- Pooled image-base screening of mitochondria with microraft isolation distinguishes pathogenic mitofusin 2 mutations.Communications biology · 2022Article
- RNA-Seq of Single Fish Cells - Seeking Out the Leukocytes Mediating Immunity in Teleost Fishes.Frontiers in immunology · 2022Review
- A technology of a different sort: microraft arrays.Lab on a chip · 2021Review
- Photodegradable Hydrogels for Rapid Screening, Isolation, and Genetic Characterization of Bacteria with Rare Phenotypes.Biomacromolecules · 2020Article
- Automated platform for cell selection and separation based on four-dimensional motility and matrix degradation.The Analyst · 2020Article
- Injection Molded Microfluidics for Establishing High-Density Single Cell Arrays in an Open Hydrogel Format.Analytical chemistry · 2020Article
- Facile Method for Fabricating Microfluidic Chip Integrated with Microwell Arrays for Cell Trapping.Micromachines · 2019Article
- Automated sensing and splitting of stem cell colonies on microraft arrays.APL bioengineering · 2019Article
- Characterization of Tensioned PDMS Membranes for Imaging Cytometry on Microraft Arrays.Analytical chemistry · 2018Article
- Intron retention enhances gene regulatory complexity in vertebrates.Genome biology · 2017Article
- Highly efficient cellular cloning using Ferro-core Micropallet Arrays.Scientific reports · 2017Article
- Single cell biology beyond the era of antibodies: relevance, challenges, and promises in biomedical research.Cellular and molecular life sciences : CMLS · 2017Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Genomic methods are used increasingly to interrogate the individual cells that compose specific tissues. However, current methods for single cell isolation struggle to phenotypically differentiate specific cells in a heterogeneous population and rely primarily on the use of fluorescent markers. Many cellular phenotypes of interest are too complex to be measured by this approach, making it difficult to connect genotype and phenotype at the level of individual cells. Here we demonstrate that microraft arrays, which are arrays containing thousands of individual cell culture sites, can be used to select single cells based on a variety of phenotypes, such as cell surface markers, cell proliferation and drug response. We then show that a common genomic procedure, RNA-seq, can be readily adapted to the single cells isolated from these rafts. We show that data generated using microrafts and our modified RNA-seq protocol compared favorably with the Fluidigm C1. We then used microraft arrays to select pancreatic cancer cells that proliferate in spite of cytotoxic drug treatment. Our single cell RNA-seq data identified several expected and novel gene expression changes associated with early drug resistance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.