ArticleScientific reports2016
Comparative Analysis of piggyBac, CRISPR/Cas9 and TALEN Mediated BAC Transgenesis in the Zygote for the Generation of Humanized SIRPA Rats.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 31 citations in OpenAlex.
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- Efficient generation of human immune system rats using human CD34Stem cell reports · 2024Article
- Revolutionizing Agriculture: Harnessing CRISPR/Cas9 for Crop Enhancement.Indian journal of microbiology · 2024Review
- Human Macrophage Long Intergenic Noncoding RNA,Arteriosclerosis, thrombosis, and vascular biology · 2023Article
- Of mice and human-specific long noncoding RNAs.Mammalian genome : official journal of the International Mammalian Genome Society · 2022Review
- In Vivo Silencing/Overexpression of lncRNAs by CRISPR/Cas System.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Advances in Genome Editing and Application to the Generation of Genetically Modified Rat Models.Frontiers in genetics · 2021Review
- Direct Readout of Neural Stem Cell Transgenesis with an Integration-Coupled Gene Expression Switch.Neuron · 2020Article
- In Vivo Analysis of Human Immune Responses in Immunodeficient Rats.Transplantation · 2020Article
- The SRG rat, a Sprague-Dawley Rag2/Il2rg double-knockout validated for human tumor oncology studies.PloS one · 2020Article
- CRISPR links to long noncoding RNA function in mice: A practical approach.Vascular pharmacology · 2019Review
- Porcine signal regulatory protein alpha binds to human CD47 to inhibit phagocytosis: Implications for human hematopoietic stem cell transplantation into severe combined immunodeficient pigs.Xenotransplantation · 2019Article
- Article
- Electroporation of mice zygotes with dual guide RNA/Cas9 complexes for simple and efficient cloning-free genome editing.Scientific reports · 2018Article
- Engineering multicellular systems: using synthetic biology to control tissue self-organization.Current opinion in biomedical engineering · 2017Article
- Advances in transgenic animal models and techniques.Transgenic research · 2017Article
- A versatile genetic tool: haploid cells.Stem cell research & therapy · 2017Review
- Review
- Animal Models for Tuberculosis in Translational and Precision Medicine.Frontiers in microbiology · 2017Review
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BAC transgenic mammalian systems offer an important platform for recapitulating human gene expression and disease modeling. While the larger body mass, and greater genetic and physiologic similarity to humans render rats well suited for reproducing human immune diseases and evaluating therapeutic strategies, difficulties of generating BAC transgenic rats have hindered progress. Thus, an efficient method for BAC transgenesis in rats would be valuable. Immunodeficient mice carrying a human SIRPA transgene have previously been shown to support improved human cell hematopoiesis. Here, we have generated for the first time, human SIRPA BAC transgenic rats, for which the gene is faithfully expressed, functionally active, and germline transmissible. To do this, human SIRPA BAC was modified with elements to work in coordination with genome engineering technologies-piggyBac, CRISPR/Cas9 or TALEN. Our findings show that piggyBac transposition is a more efficient approach than the classical BAC transgenesis, resulting in complete BAC integration with predictable end sequences, thereby permitting precise assessment of the integration site. Neither CRISPR/Cas9 nor TALEN increased BAC transgenesis. Therefore, an efficient generation of human SIRPA transgenic rats using piggyBac opens opportunities for expansion of humanized transgenic rat models in the future to advance biomedical research and therapeutic applications.
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