ArticleInsect biochemistry and molecular biology2016
Determination of juvenile hormone titers by means of LC-MS/MS/MS and a juvenile hormone-responsive Gal4/UAS system in Aedes aegypti mosquitoes.
Article in Insect biochemistry and molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- Lysine Triggers Acute Oviposition by Activating the 20E-ETH-JH Signaling Cascade inInternational journal of molecular sciences · 2026Article
- Juvenile hormone-mediated chromatin remodelling by cumulative action of downstream transcription factors, Hairy and Krüppel homolog 1.Communications biology · 2026Article
- Juvenile hormone induces phosphorylation of insulin/insulin-like growth factor signaling proteins in previtellogenic Aedes aegypti mosquitoes.Insect science · 2025Article
- Membrane-localized MET engages PVR to mediate extranuclear juvenile hormone signaling inProceedings of the National Academy of Sciences of the United States of America · 2025Article
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- Lysyl-tRNA synthetase orchestrates translation and hormone signaling during reproduction in Aedes aegypti mosquitoes.Communications biology · 2025Article
- Article
- Sex- and caste-specific developmental responses to juvenile hormone in an ant with maternal caste determination.The Journal of experimental biology · 2024Article
- An 11-point time course midgut transcriptome across 72 h after bloodfeeding provides detailed temporal resolution of transcript expression in the arbovirus vector,Genome research · 2023Article
- miR-309a is a regulator of ovarian development in the oriental fruit fly Bactrocera dorsalis.PLoS genetics · 2022Article
- A transcriptomic atlas ofeLife · 2022Article
- Article
- E93 confers steroid hormone responsiveness of digestive enzymes to promote blood meal digestion in the midgut of the mosquito Aedes aegypti.Insect biochemistry and molecular biology · 2021Article
- The ecdysone-induced protein 93 is a key factor regulating gonadotrophic cycles in the adult female mosquitoProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Steroid hormone ecdysone deficiency stimulates preparation for photoperiodic reproductive diapause.PLoS genetics · 2021Article
- Approaches and Tools to Study the Roles of Juvenile Hormones in Controlling Insect Biology.Insects · 2020Review
- Hemocyte-targeted gene expression in the female malaria mosquito using the hemolectin promoter from Drosophila.Insect biochemistry and molecular biology · 2020Article
- Blood feeding activates the vitellogenic stage of oogenesis in the mosquito Aedes aegypti through inhibition of glycogen synthase kinase 3 by the insulin and TOR pathways.Developmental biology · 2019Article
- Synergistic action of the transcription factors Krüppel homolog 1 and Hairy in juvenile hormone/Methoprene-tolerant-mediated gene-repression in the mosquito Aedes aegypti.PLoS genetics · 2019Article
- Juvenile hormone-regulated alternative splicing of theProceedings of the National Academy of Sciences of the United States of America · 2018Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
In anautogenous mosquitoes, juvenile hormone III (JH) plays an essential role in female post-eclosion (PE) development, preparing them for subsequent blood feeding and egg growth. We re-examined the JH titer during the reproductive cycle of female Aedes aegypti mosquitoes. Using liquid chromatography coupled with triple tandem mass spectrometry (LC-MS/MS/MS), we have shown that it reaches its peak at 48-54 h PE in the female hemolymph and at 72 h PE in whole body extracts. This method represents an effective assay for determination of JH titers. The 2.1-kb 5' promoter region of the Early Trypsin (ET) gene, which is specifically expressed in the female midgut under the control of JH during the PE phase, was utilized to genetically engineer the Ae. aegypti mosquito line with the ET-Gal4 activator. We then established the ET-GAL4>UAS-enhanced green fluorescent protein (EGFP) system in Ae. aegypti. In ET-Gal4>UAS-EGFP female mosquitoes, the intensity of the midgut-specific EGFP signal was observed to correspond to the ET gene transcript level and follow the JH titer during the PE phase. The EGFP signal and the EGFP transcript level were significantly diminished in midguts of transgenic female mosquitoes after RNA interference depletion of the JH receptor Methoprene-tolerant (Met), providing evidence of the control of ET gene expression by Met. Topical JH application caused premature enhancement of the EGFP signal and the EGFP transcript level in midguts of newly eclosed ET-Gal4>UAS-EGFP female mosquitoes, in which endogenous JH titer is still low. Hence, this novel ET-Gal4>UAS system permits JH-dependent gene overexpression in the midgut of Ae. aegypti female mosquitoes prior to a blood meal.
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