ArticlePloS one2016
Testis-Specific Bb8 Is Essential in the Development of Spermatid Mitochondria.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 28 citations in OpenAlex.
- Review
- Analysis of Transcript Expression and Core Promoter DNA Sequences of Brain, Adipose Tissues and Testis in Human and Fruit Fly.International journal of molecular sciences · 2025Article
- A comparison of spermatogenesis between flies and men-conserved processes of male gamete production.Human reproduction update · 2025Review
- Single-cell consequences of X-linked meiotic drive in stalk-eyed flies.PLoS genetics · 2025Article
- Multi-omics integration reveals Vha68-3 as a testicular aging-specific factor that coordinates spermatid elongation through mitochondrial metabolic homeostasis.Cellular & molecular biology letters · 2025Article
- Deficiency ofInternational journal of molecular sciences · 2024Article
- Mitochondrial Differentiation during Spermatogenesis: Lessons fromInternational journal of molecular sciences · 2024Review
- Germline and Somatic Cell Syncytia in Insects.Results and problems in cell differentiation · 2024Review
- Structure and molecular basis of spermatid elongation in theOpen biology · 2023Review
- Article
- Article
- A comparative analysis of fruit fly and human glutamate dehydrogenases inFrontiers in cell and developmental biology · 2023Article
- Analytical approach for specific populations at single-cell resolution: insights for ND-42 mediated mitochondrial derivative function during spermatid elongation.American journal of clinical and experimental immunology · 2023Article
- Comparative Ubiquitome Analysis Reveals Deubiquitinating Effects Induced byInternational journal of molecular sciences · 2022Article
- Condition-dependent functional shift of twoAutophagy · 2021Article
- The tumor suppressor archipelago E3 ligase is required for spermatid differentiation in Drosophila testis.Scientific reports · 2021Article
- Microtubule Organizing Centers Contain Testis-Specific γ-TuRC Proteins in Spermatids of Drosophila.Frontiers in cell and developmental biology · 2021Article
- Wampa is a dynein subunit required for axonemal assembly and male fertility in Drosophila.Developmental biology · 2020Article
- A Genetic Screen Using theG3 (Bethesda, Md.) · 2019Article
- Sperm-Leucylaminopeptidases are required for male fertility as structural components of mitochondrial paracrystalline material in Drosophila melanogaster sperm.PLoS genetics · 2019Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mitochondria are essential organelles of developing spermatids in Drosophila, which undergo dramatic changes in size and shape after meiotic division, where mitochondria localized in the cytoplasm, migrate near the nucleus, aggregate, fuse and create the Nebenkern. During spermatid elongation the two similar mitochondrial derivatives of the Nebenkern start to elongate parallel to the axoneme. One of the elongated mitochondrial derivatives starts to lose volume and becomes the minor mitochondrial derivative, while the other one accumulates paracrystalline and becomes the major mitochondrial derivative. Proteins and intracellular environment that are responsible for cyst elongation and paracrystalline formation in the major mitochondrial derivative need to be identified. In this work we investigate the function of the testis specific big bubble 8 (bb8) gene during spermatogenesis. We show that a Minos element insertion in bb8 gene, a predicted glutamate dehydrogenase, causes recessive male sterility. We demonstrate bb8 mRNA enrichment in spermatids and the mitochondrial localisation of Bb8 protein during spermatogenesis. We report that megamitochondria develop in the homozygous mutant testes, in elongating spermatids. Ultrastructural analysis of the cross section of elongated spermatids shows enlarged mitochondria and the production of paracrystalline in both major and minor mitochondrial derivatives. Our results suggest that the Bb8 protein and presumably glutamate metabolism has a crucial role in the normal development and establishment of the identity of the mitochondrial derivatives during spermatid elongation.
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